{"product_id":"reps2-antibody-ralbp1-associated-eps-domain-containing-protein-2-bha17136107","title":"REPS2 Antibody \/ RalBP1-associated Eps domain-containing protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eREPS2 Antibody \/ RalBP1-associated Eps domain-containing protein 2 is a anti-REPS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e REPS2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eREPS2 antibody detects RalBP1-associated Eps domain-containing protein 2, a signaling adaptor that mediates endocytosis and receptor trafficking. The UniProt recommended name is RalBP1-associated Eps domain-containing protein 2 (REPS2). This cytoplasmic protein functions in clathrin-mediated endocytosis, growth factor receptor internalization, and signal transduction downstream of small GTPases such as RalA and RalB.\u003cbr\u003e\u003cbr\u003eFunctionally, REPS2 antibody identifies a 639-amino-acid protein containing EH (Eps15 homology) domains that mediate interactions with endocytic machinery proteins including Eps15, RalBP1, and AP-2. REPS2 contributes to the internalization of epidermal growth factor receptor (EGFR) and other tyrosine kinase receptors, influencing receptor recycling and downregulation. It also participates in the regulation of cytoskeletal dynamics and cell adhesion through Ral GTPase-dependent pathways.\u003cbr\u003e\u003cbr\u003eThe REPS2 gene is located on chromosome Xp22.3 and is expressed in epithelial tissues, neurons, and endocrine glands. In addition to its role in endocytosis, REPS2 modulates insulin signaling, exocytosis, and vesicular trafficking, providing a link between receptor activation and intracellular transport systems.\u003cbr\u003e\u003cbr\u003ePathologically, aberrant REPS2 expression has been implicated in prostate cancer, breast cancer, and neurological disorders. Overexpression promotes cancer cell migration and survival by sustaining receptor tyrosine kinase signaling, while loss of REPS2 can impair receptor internalization and lead to altered growth factor responsiveness. Research using REPS2 antibody supports studies in membrane trafficking, cell signaling, and tumor progression.\u003cbr\u003e\u003cbr\u003eREPS2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect endocytic adaptor proteins. \u003cbr\u003e\u003cbr\u003eStructurally, RalBP1-associated Eps domain-containing protein 2 contains N-terminal EH domains, central coiled-coil motifs, and proline-rich regions that mediate protein-protein interactions. These domains form scaffolds for complex assembly with signaling and endocytic components. This antibody enables the analysis of REPS2's role in receptor-mediated endocytosis and signaling modulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=REPS2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=REPS2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=REPS2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/REPS2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=REPS2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318741357,"sku":"FY13205","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_150be0c2-29e6-434c-9b0c-95f8c98a4a3f.jpg?v=1782237097","url":"https:\/\/www.ebiohippo.com\/products\/reps2-antibody-ralbp1-associated-eps-domain-containing-protein-2-bha17136107","provider":"BioHippo","version":"1.0","type":"link"}