{"product_id":"rest-antibody-re1-silencing-transcription-factor-bha17135919","title":"REST Antibody \/ RE1-silencing transcription factor","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eREST Antibody \/ RE1-silencing transcription factor is a anti-REST Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e REST\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eREST antibody detects RE1-silencing transcription factor, a zinc finger DNA-binding protein that represses neuronal gene expression in non-neuronal cells. The UniProt recommended name is RE1-silencing transcription factor (REST). Also known as neuron-restrictive silencer factor (NRSF), this protein plays a pivotal role in epigenetic regulation of gene expression by binding to RE1\/NRSE motifs and recruiting chromatin-modifying complexes.\u003cbr\u003e\u003cbr\u003eFunctionally, REST antibody identifies a 1097-amino-acid nuclear protein that suppresses transcription by forming repressive chromatin structures. REST recognizes RE1 sites present in the promoters of neuronal genes and recruits corepressors such as CoREST, mSin3, and histone deacetylases (HDAC1\/2). These interactions promote chromatin condensation and transcriptional silencing, preventing inappropriate neuronal gene expression in non-neuronal tissues.\u003cbr\u003e\u003cbr\u003eThe REST gene is located on chromosome 4q12 and encodes a multi-zinc finger transcription factor with eight C2H2 zinc finger motifs that mediate sequence-specific DNA binding. REST acts as a master regulator of neurogenesis, ensuring proper timing of neuronal differentiation. During development, REST levels decline in differentiating neurons, allowing the expression of genes required for synaptic function, ion channel activity, and neurotransmitter release.\u003cbr\u003e\u003cbr\u003eIn addition to developmental regulation, REST participates in neural plasticity, stem cell maintenance, and stress response pathways. Dysregulation of REST has been linked to neurological disorders, including epilepsy, Huntington's disease, and Alzheimer's disease. Elevated REST expression in non-neuronal cancers such as medulloblastoma and neuroblastoma suggests a role in tumorigenesis through repression of differentiation pathways.\u003cbr\u003e\u003cbr\u003eREST antibody is widely used in neuroscience, epigenetics, and cancer research. It is suitable for chromatin immunoprecipitation (ChIP), immunoblotting, and immunofluorescence to study REST localization, DNA binding, and interaction networks. This antibody aids in exploring transcriptional silencing mechanisms and neurodevelopmental regulation. In oncology, REST serves as a biomarker of altered differentiation and epigenetic reprogramming.\u003cbr\u003e\u003cbr\u003eStructurally, REST contains an N-terminal repression domain that recruits mSin3-HDAC complexes and a C-terminal domain that binds CoREST and associated enzymes such as LSD1. REST-mediated repression involves coordinated histone deacetylation and demethylation, establishing a transcriptionally repressive chromatin environment.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=REST - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=REST - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=REST - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/REST - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=REST+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309304173,"sku":"FY13017","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_56d7fe56-6f8d-450f-9a39-26caa8795fdc.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/rest-antibody-re1-silencing-transcription-factor-bha17135919","provider":"BioHippo","version":"1.0","type":"link"}