{"product_id":"rfx4-antibody-regulatory-factor-x4-bha17135346","title":"RFX4 Antibody \/ Regulatory factor X4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRFX4 Antibody \/ Regulatory factor X4 is a anti-RFX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RFX4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRFX4 antibody recognizes regulatory factor X4, a transcription factor belonging to the RFX family of winged-helix DNA-binding proteins. RFX4 binds to X-box motifs in promoter regions and regulates the transcription of numerous genes involved in development, cell differentiation, and ciliary function. Within the brain, RFX4 plays an essential role in the differentiation of neural progenitors and the maintenance of ventricular zone organization. It is expressed predominantly in the central nervous system, with enrichment in the hypothalamus and cerebral cortex. The RFX4 antibody is widely used to investigate its role in neurodevelopment, ciliogenesis, and transcriptional regulation of genes associated with signaling and metabolism.\u003cbr\u003e\u003cbr\u003eRFX4 is encoded by the RFX4 gene located on human chromosome 12q23.1. Alternative splicing of this gene produces several isoforms, including RFX4_v1, RFX4_v2, and RFX4_v3, each differing in transcriptional activity and tissue expression. The protein contains a conserved DNA-binding domain characteristic of RFX family members, as well as a dimerization domain that allows heterodimer formation with other RFX proteins such as RFX2 or RFX3. These interactions contribute to complex transcriptional control of cilia-related genes, making RFX4 a central node in the regulation of motile and primary cilia biogenesis.\u003cbr\u003e\u003cbr\u003eThe RFX4 antibody has been instrumental in studies linking gene expression patterns to developmental brain disorders. Loss-of-function mutations or altered RFX4 expression have been associated with congenital hydrocephalus, holoprosencephaly, and various neurodevelopmental syndromes. Mouse models deficient in Rfx4 show severe forebrain malformations and altered neuronal differentiation, demonstrating its crucial role in brain patterning. Beyond the CNS, RFX4 contributes to the regulation of genes expressed in reproductive and endocrine tissues, including those controlling gonadotropin-releasing hormone production and pituitary signaling. Researchers use this antibody for western blot, immunohistochemistry, and immunofluorescence to monitor its expression during neurogenesis and in cultured cells undergoing differentiation.\u003cbr\u003e\u003cbr\u003eIn experimental systems, RFX4 localizes mainly to the nucleus, where it binds target promoters and interacts with co-regulatory proteins such as CREB-binding protein (CBP) and EP300. Studies using chromatin immunoprecipitation and transcriptome analyses have revealed that RFX4 controls transcriptional programs tied to ciliary trafficking, cell polarity, and synaptic development.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RFX4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RFX4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RFX4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RFX4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RFX4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288299885,"sku":"FY12443","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_35e50263-8ea5-4f2d-8699-ea618c89b787.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/rfx4-antibody-regulatory-factor-x4-bha17135346","provider":"BioHippo","version":"1.0","type":"link"}