{"product_id":"rfxank-antibody-regulatory-factor-x-associated-ankyrin-containing-protein-bha17136096","title":"RFXANK Antibody \/ Regulatory factor X-associated ankyrin-containing protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRFXANK Antibody \/ Regulatory factor X-associated ankyrin-containing protein is a anti-RFXANK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RFXANK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRFXANK antibody detects Regulatory factor X-associated ankyrin-containing protein, a transcriptional regulator essential for major histocompatibility complex (MHC) class II gene expression. The UniProt recommended name is Regulatory factor X-associated ankyrin-containing protein (RFXANK). This nuclear protein forms part of the RFX complex with RFX5 and RFXAP, which binds to X-box promoter elements and activates MHC class II transcription.\u003cbr\u003e\u003cbr\u003eFunctionally, RFXANK antibody identifies a 260-amino-acid nuclear protein containing multiple ankyrin repeats that mediate protein-protein interactions within the RFX complex. RFXANK acts as a scaffold for assembling the class II transactivator (CIITA) and other coactivators at MHC class II promoters, enabling antigen-presenting cells to express HLA-D gene products critical for immune recognition.\u003cbr\u003e\u003cbr\u003eThe RFXANK gene is located on chromosome 19p12 and is highly expressed in antigen-presenting cells such as B lymphocytes, macrophages, and dendritic cells. Its function is indispensable for adaptive immune activation and T-cell mediated responses.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in RFXANK cause bare lymphocyte syndrome type II (BLS II), a severe immunodeficiency characterized by loss of MHC class II expression and defective antigen presentation. Research using RFXANK antibody supports studies in transcriptional regulation, immunodeficiency, and antigen presentation biology.\u003cbr\u003e\u003cbr\u003eRFXANK antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcriptional regulators involved in immune gene activation. \u003cbr\u003e\u003cbr\u003eStructurally, Regulatory factor X-associated ankyrin-containing protein contains tandem ankyrin repeats and a nuclear localization signal that mediate its assembly into DNA-binding complexes. This antibody enables investigation of RFXANK's role in immune gene transcription and MHC regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RFXANK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RFXANK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RFXANK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RFXANK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RFXANK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314841965,"sku":"FY13194","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_310d0ddf-f0ed-45e0-8a50-38606760fd6d.jpg?v=1782237109","url":"https:\/\/www.ebiohippo.com\/products\/rfxank-antibody-regulatory-factor-x-associated-ankyrin-containing-protein-bha17136096","provider":"BioHippo","version":"1.0","type":"link"}