{"product_id":"rgl3-antibody-ral-guanine-nucleotide-dissociation-stimulator-like-3-bha17136220","title":"RGL3 Antibody \/ Ral guanine nucleotide dissociation stimulator-like 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRGL3 Antibody \/ Ral guanine nucleotide dissociation stimulator-like 3 is a anti-RGL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RGL3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRGL3 antibody recognizes Ral guanine nucleotide dissociation stimulator-like 3, a cytoplasmic guanine nucleotide exchange factor (GEF) encoded by the RGL3 gene on chromosome 19q13.33. RGL3 is a member of the RalGDS-like family of Ras effector proteins that mediate cross-talk between Ras and Ral small GTPase signaling pathways. RGL3 functions as a guanine nucleotide exchange factor that activates RalA and RalB by promoting the exchange of GDP for GTP, thus regulating vesicle trafficking, cytoskeletal organization, and cell proliferation.\u003cbr\u003e\u003cbr\u003eRGL3 antibody identifies a multifunctional signaling adaptor that localizes primarily to the cytoplasm and cell membrane, where it interacts with active GTP-bound Ras. Through its Ras-binding domain (RBD) and pleckstrin homology (PH) domain, RGL3 serves as a molecular bridge linking Ras activation to downstream Ral-mediated signaling. This pathway plays critical roles in exocytosis, endocytosis, and integrin-dependent cell migration. Structural analyses reveal that RGL3 possesses distinct domains for Ras and phospholipid interactions, allowing it to coordinate spatial and temporal aspects of Ral signaling.\u003cbr\u003e\u003cbr\u003eFunctionally, RGL3 participates in pathways controlling cytoskeletal remodeling and membrane trafficking. Activated RGL3 promotes the assembly of Ral effector complexes involved in vesicle tethering and actin reorganization. In epithelial cells, it contributes to junction formation and cell polarity maintenance. The Ral signaling axis, modulated by RGL3, influences diverse cellular functions including autophagy, exosome release, and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eRGL3 is transcriptionally regulated by growth factors and mitogenic stimuli, positioning it as an important node in the Ras-MAPK signaling network. Dysregulation of RGL3 expression or activity has been linked to cancer progression, metastasis, and neurological disorders. Overactivation of the Ral GTPase pathway through RGL3 can enhance tumor cell migration and invasion, particularly in pancreatic and lung cancers. Conversely, loss of RGL3 function can impair vesicular transport and disrupt cell adhesion dynamics.\u003cbr\u003e\u003cbr\u003eAt the pathway level, RGL3 participates in Ras and Ral GTPase signaling cascades, which intersect with PI3K and MAPK pathways to control cell survival and differentiation. Evolutionarily, RGL3 belongs to the RalGDS family of guanine nucleotide exchange factors, sharing homology with RGL1, RGL2, and RalGPS proteins. The protein's regulatory domains ensure precise spatiotemporal activation of Ral GTPases in response to external stimuli.\u003cbr\u003e\u003cbr\u003eImmunohistochemical analysis using RGL3 antibody shows cytoplasmic and membrane localization in epithelial and neuronal cells, consistent with its role in Ras-dependent signaling. RGL3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RGL3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RGL3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RGL3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RGL3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RGL3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322083693,"sku":"FY13318","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e7b26e00-cf5a-4fbd-83ca-2af0a793db1a.jpg?v=1782237108","url":"https:\/\/www.ebiohippo.com\/products\/rgl3-antibody-ral-guanine-nucleotide-dissociation-stimulator-like-3-bha17136220","provider":"BioHippo","version":"1.0","type":"link"}