{"product_id":"rhot2-antibody-rho-gtpase-2-miro2-bha17135261","title":"RHOT2 Antibody \/ Rho GTPase 2 \/ MIRO2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRHOT2 Antibody \/ Rho GTPase 2 \/ MIRO2 is a anti-RHOT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RHOT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe RHOT2 antibody targets Mitochondrial Rho GTPase 2, a small GTP-binding protein encoded by the RHOT2 gene. Also known as Miro2, this protein is anchored to the outer mitochondrial membrane and regulates mitochondrial trafficking, dynamics, and calcium signaling. Mitochondrial Rho GTPase 2 interacts with adaptors such as TRAK1 and kinesin motor proteins to mediate mitochondrial transport along microtubules. The RHOT2 antibody enables detailed analysis of mitochondrial movement, morphology, and positioning within cells under physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003eMitochondrial Rho GTPase 2 is part of the Miro family, which also includes RHOT1 (Miro1). Both proteins contain two GTPase domains and two calcium-binding EF-hand motifs that sense cytosolic calcium levels to control organelle motility. Under elevated calcium conditions, RHOT2 disengages mitochondria from microtubule motors, halting transport and localizing organelles near energy-demanding sites. The RHOT2 antibody is essential for studying these regulatory events and mapping mitochondrial dynamics during cell stress, differentiation, and neuronal signaling.\u003cbr\u003e\u003cbr\u003eDefects in RHOT2-mediated transport are linked to neurodegenerative disorders such as Parkinson's disease and Charcot-Marie-Tooth disease. Impaired mitochondrial motility can disrupt energy distribution, leading to axonal degeneration and synaptic dysfunction. The RHOT2 antibody provides a means to measure protein expression in neuronal tissues and to investigate molecular mechanisms that couple mitochondrial movement with cellular signaling pathways. By examining Mitochondrial Rho GTPase 2 expression, researchers gain insight into how mitochondrial positioning influences metabolism and neuroprotection.\u003cbr\u003e\u003cbr\u003eIn addition to its transport role, Mitochondrial Rho GTPase 2 contributes to mitochondrial quality control and mitophagy. It interacts with the E3 ubiquitin ligase Parkin and participates in PINK1-Parkin-mediated degradation of damaged mitochondria. The RHOT2 antibody allows visualization of these processes in cellular models of mitochondrial dysfunction, facilitating research into the molecular pathology of Parkinsonian syndromes and mitochondrial diseases. Furthermore, altered RHOT2 expression has been associated with metabolic stress and cancer cell survival, underscoring its broader physiological importance.\u003cbr\u003e\u003cbr\u003eThe RHOT2 antibody is suitable for use in western blotting, immunofluorescence microscopy, and immunohistochemistry. It produces a distinct mitochondrial staining pattern, enabling analysis of organelle distribution and morphology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RHOT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RHOT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RHOT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RHOT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RHOT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285449069,"sku":"FY12358","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_89d20740-682d-4fe6-8196-ae50377b20e3.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/rhot2-antibody-rho-gtpase-2-miro2-bha17135261","provider":"BioHippo","version":"1.0","type":"link"}