{"product_id":"rig-antibody-protein-regulated-in-glioma-bha17135920","title":"RIG Antibody \/ Protein regulated in glioma","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRIG Antibody \/ Protein regulated in glioma is a anti-RIG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RIG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRIG antibody detects Protein regulated in glioma (RIG), a small nuclear protein of approximately 12 kDa composed of 110 amino acids. This protein was originally identified in glioma cell lines, where its expression correlated with tumor cell growth and differentiation state. The UniProt recommended name is Protein regulated in glioma (RIG). Unlike RIG-I (the 102 kDa DExD\/H-box helicase involved in antiviral signaling), this RIG protein is a distinct nuclear factor believed to participate in transcriptional regulation and RNA metabolism within glial and neuronal cells.\u003cbr\u003e\u003cbr\u003eRIG localizes predominantly to the nucleus and may function as a DNA- or RNA-binding protein that modulates transcriptional output under conditions of metabolic or proliferative stress. Its small size and sequence composition suggest that it acts as an auxiliary nuclear factor rather than a canonical enzyme. Expression analyses from glioma and astrocytoma models indicate that RIG levels can fluctuate during tumor progression, possibly reflecting changes in transcriptional activity, cell cycle control, or differentiation signaling. Overexpression of RIG in some glioma-derived lines has been linked to enhanced proliferation and resistance to apoptotic stimuli, whereas reduced expression can coincide with altered cell morphology or loss of growth capacity.\u003cbr\u003e\u003cbr\u003eThe RIG gene encodes a compact protein that may influence chromatin accessibility or RNA processing. It is expressed in several tissues but shows highest abundance in neural and glial populations. Its amino acid sequence contains motifs compatible with nuclear localization and RNA recognition, supporting a role in coordinating transcriptional output with cellular stress or growth cues. Although the detailed mechanisms remain under investigation, RIG is thought to act as a nuclear modulator of gene expression relevant to glioma pathogenesis.\u003cbr\u003e\u003cbr\u003eAt the cellular level, RIG expression has been reported to respond to external stimuli such as serum withdrawal, oxidative stress, and exposure to differentiation agents. These findings suggest that RIG may help cells adapt transcriptionally to environmental or developmental cues. In glioma models, its upregulation is often associated with actively dividing cells, consistent with a role in sustaining nuclear RNA metabolism and growth-associated gene expression programs.\u003cbr\u003e\u003cbr\u003eRIG antibody provides a specific reagent for detecting this 12 kDa nuclear protein in cultured cells and tissue extracts. It supports research focused on glioma biology, transcriptional regulation, and nuclear protein function. The antibody can aid in identifying RIG expression patterns in tumor versus normal brain tissue and in exploring nuclear pathways involved in glial differentiation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RIG - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RIG - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RIG - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RIG - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RIG+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309762925,"sku":"FY13018","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_855b123d-04d8-4bce-a52f-9a697d7256a1.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/rig-antibody-protein-regulated-in-glioma-bha17135920","provider":"BioHippo","version":"1.0","type":"link"}