{"product_id":"rims1-antibody-regulating-synaptic-membrane-exocytosis-protein-1-bha17135690","title":"RIMS1 Antibody \/ Regulating synaptic membrane exocytosis protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRIMS1 Antibody \/ Regulating synaptic membrane exocytosis protein 1 is a anti-RIMS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RIMS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRIMS1 antibody detects Regulating synaptic membrane exocytosis protein 1, a presynaptic scaffold protein essential for synaptic vesicle priming and neurotransmitter release. Encoded by the RIMS1 gene on chromosome 6q13, this protein belongs to the RIM (Regulating synaptic membrane exocytosis) family and functions as a central organizer of the active zone at presynaptic terminals. RIMS1 coordinates synaptic vesicle docking with calcium-triggered exocytosis by linking voltage-gated calcium channels to synaptic vesicle proteins and cytoskeletal components.\u003cbr\u003e\u003cbr\u003eStructurally, RIMS1 contains zinc finger, PDZ, C2A, and C2B domains that mediate interactions with Munc13, RAB3A, ELKS, liprins, and calcium channels. Through these interactions, RIMS1 facilitates vesicle tethering, calcium channel clustering, and efficient neurotransmitter release. It localizes to the cytoplasmic face of active zones in excitatory and inhibitory neurons, where it serves as a molecular hub integrating presynaptic signaling and vesicle dynamics.\u003cbr\u003e\u003cbr\u003eThe RIMS1 antibody is used in neurobiology and synaptic physiology research to study synaptic vesicle trafficking, neurotransmitter release, and presynaptic organization. Western blot analysis typically identifies a 175-180 kilodalton band corresponding to RIMS1, while immunofluorescence reveals punctate staining at presynaptic terminals. This antibody supports studies of neuronal communication, synaptic plasticity, and calcium-dependent exocytosis.\u003cbr\u003e\u003cbr\u003eMutations in RIMS1 are associated with cone-rod dystrophy and autism spectrum disorders, highlighting its importance in both neuronal development and sensory transmission. RIMS1 deficiency disrupts synaptic organization and impairs neurotransmitter release efficiency. The RIMS1 antibody is a key reagent for analyzing synaptic machinery and presynaptic protein complexes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RIMS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RIMS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RIMS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RIMS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RIMS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047301734765,"sku":"FY12788","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a0734f77-eab0-48e5-8c7c-4b6a82a06748.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/rims1-antibody-regulating-synaptic-membrane-exocytosis-protein-1-bha17135690","provider":"BioHippo","version":"1.0","type":"link"}