{"product_id":"ring1-antibody-polycomb-complex-protein-ring1-bha17135997","title":"RING1 Antibody \/ Polycomb complex protein RING1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRING1 Antibody \/ Polycomb complex protein RING1 is a anti-RING1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RING1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRING1 antibody detects E3 ubiquitin-protein ligase RING1, a chromatin-associated enzyme that catalyzes histone ubiquitination and transcriptional repression. The UniProt recommended name is E3 ubiquitin-protein ligase RING1 (RING1). This enzyme is a key component of the Polycomb repressive complex 1 (PRC1), which regulates gene silencing during development and differentiation.\u003cbr\u003e\u003cbr\u003eFunctionally, RING1 antibody identifies a 406-amino-acid nuclear enzyme containing a RING finger domain that mediates E3 ubiquitin ligase activity. RING1 monoubiquitinates histone H2A at lysine 119, a hallmark modification that represses transcription of developmental genes. It forms a heterodimer with RING1B or BMI1, together constituting the catalytic core of PRC1. This activity ensures stable repression of genes controlling cell fate and pluripotency.\u003cbr\u003e\u003cbr\u003eThe RING1 gene is located on chromosome 6p22.3 and is broadly expressed, with high levels in embryonic and stem cell populations. RING1 functions as an epigenetic regulator, linking chromatin compaction to long-term gene silencing. Its activity is modulated by PRC2-mediated H3K27 methylation, which recruits PRC1 to specific genomic loci.\u003cbr\u003e\u003cbr\u003ePathologically, deregulated RING1 expression contributes to developmental abnormalities and cancer. Overexpression of PRC1 components, including RING1, enhances chromatin repression and promotes oncogenic transformation. Conversely, loss of RING1 impairs differentiation and disrupts gene silencing. Research using RING1 antibody aids studies in epigenetic regulation, chromatin structure, and stem cell biology.\u003cbr\u003e\u003cbr\u003eRING1 antibody is suitable for western blotting, immunoprecipitation, and chromatin immunoprecipitation to detect PRC1 complex components and histone modifications. \u003cbr\u003e\u003cbr\u003eStructurally, RING1 possesses an N-terminal RING finger motif coordinating two zinc ions essential for ubiquitin transfer. It interacts with E2 ubiquitin-conjugating enzymes to catalyze substrate modification. This antibody enables analysis of RING1's central role in chromatin remodeling and transcriptional repression through histone ubiquitination.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RING1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RING1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RING1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RING1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RING1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311991149,"sku":"FY13095","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_63744a7d-da11-43be-bb22-4f43cdc96f76.jpg?v=1782237110","url":"https:\/\/www.ebiohippo.com\/products\/ring1-antibody-polycomb-complex-protein-ring1-bha17135997","provider":"BioHippo","version":"1.0","type":"link"}