{"product_id":"rnase-hii-2-u-mu-l-bhz20800035","title":"RNase HII (2 U\/μL)","description":"\u003cp style=\"font-weight: 400;\"\u003eRNase HII is a ribonuclease internal enzyme obtained from the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ePyrococcus abyssi\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eand recombinantly expressed in\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. Coli\u003c\/em\u003e. It recognizes the DNA-rN-DNA\/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.022%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.2587%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 82.4157%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14539ES80\/14539ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.2587%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 82.4157%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e50 U \/ 250 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.2587%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSpecies\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 82.4157%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003e\u003ci\u003ePyrococcus abyssi, P.a.\u003c\/i\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.2587%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eActivity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 82.4157%;\"\u003e\n\u003cp data-pm-slice=\"1 1 []\"\u003e\u003cspan\u003eOne unit is defined as the amount of enzyme necessary to nick 1 nmol of synthetic, single-rC-containing duplex substrate per minute at 70°C in 10 mM Tris, 50 mM NaCl, 0.01% Triton X-100, 10 µg\/ml BSA, 4 mM MgCl2. Depending on the application, enzyme usage range is 2−200 mU per reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.061%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 42.5914%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 30.5405%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14539ES50 (50U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.1861%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14539ES72 (250U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 42.5914%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase HII（2 U\/μL）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 30.5405%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.1861%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e125 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at-25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eProduct Application\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eDetection by LAMP with high-sensitivity probes.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eRNase HII-dependent PCR (rhPCR).\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eRemoval of mismatched ribonucleotides formed during polymerase chain reaction.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eDegradation of the RNA portion of Okazaki fragments.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThis product is for research use only.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003ePlease operate with lab coats and disposable gloves, for your safety.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eFigures\u003c\/h3\u003e\n\u003ch4\u003e\n\u003cstrong\u003e\u003cem\u003e\u003cb\u003e\u003ci\u003e1. E. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/i\u003e\u003c\/b\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cb\u003egenomic DNA residue \u0026lt; 0.5 copies\/100 U\u003c\/b\u003e\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cp\u003eDetection of\u003cem\u003e\u003ci\u003e E. coli\u003c\/i\u003e\u003c\/em\u003e genomic DNA residue in different batches of RNase HII showed that the host genomic DNA residue of YEASEN's RNase HII is far below 0.5 copies\/100 U.\u003c\/p\u003e\n\u003cdiv\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_83967ea2-0437-491a-9d03-226959eb176c_1024x1024.png?v=1731651670\"\u003e\u003c\/div\u003e\n\u003cp\u003eFig 4. Detection of\u003cem\u003e\u003ci\u003e E. coli\u003c\/i\u003e\u003c\/em\u003e genomic DNA residue\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cb\u003e2. 95°C Heat Resistance Test\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003eAfter heating RNase HII at 95°C for 0 to 45 minutes, the enzyme activity of RNase HII was tested. The results indicated that there was almost no loss of enzyme activity in YEASEN's RNase HII even after 45 minutes of heating.\u003c\/p\u003e\n\u003cdiv\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_faa57b24-fdd7-4337-9617-87f3b5c2e4a5_600x600.png?v=1731651670\"\u003e\u003c\/div\u003e\n\u003cp\u003eFig 5. 95°C heat resistance test\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cb\u003e3. No Exonuclease., Nicking Enzyme, or RNase Residue (1 U Dose)\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003eIncubating 1 U of different batches of RNase HII with their respective substrates DNA\/RNA at 37°C for 4 hours, the results of agarose gel electrophoresis indicated that there was no exonuclease, nicking enzyme, or RNase residue in RNase HII.\u003c\/p\u003e\n\u003cdiv\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_fa766c8e-c681-4b38-950e-c9d701284766_600x600.png?v=1731651670\"\u003e\u003c\/div\u003e\n\u003cp\u003eFig 6. Detection of exonuclease, nicking enzyme, and RNase residue\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 550;\"\u003eDocuments:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14539_Manual_Ver.EN20240116.pdf?v=1735543924\"\u003eManuals\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294150025581,"sku":"14539ES72","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4_1024x1024_183b8615-867e-4db1-9fb0-b907f85d1339.png?v=1778101062","url":"https:\/\/www.ebiohippo.com\/products\/rnase-hii-2-u-mu-l-bhz20800035","provider":"BioHippo","version":"1.0","type":"link"}