{"product_id":"rnase-hii-glycerol-free-2-u-mu-l-bhz20800036","title":"RNase HII, Glycerol-free (2 U\/μL)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp style=\"font-weight: 400;\"\u003eRNase HII is a ribonuclease internal enzyme obtained from the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ePyrococcus abyssi\u003c\/em\u003e and recombinantly expressed in\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. Coli.\u003c\/em\u003e It recognizes the DNA-rN-DNA\/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems. This product does not contain glycerol and is specifically used for the preparation of freeze-dried reagents.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14541ES80\/14541ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1 KU \/10 KU\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14541ES80  \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14541ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase HII, Glycerol-free (2 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5× 1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at\u003cspan\u003e \u003c\/span\u003e2～8℃ for 6 mouths.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eProduct Application\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eDetection by LAMP with high-sensitivity probes.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eRNase HII-dependent PCR (rhPCR).\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eRemoval of mismatched ribonucleotides formed during polymerase chain reaction.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eDegradation of the RNA portion of Okazaki fragments.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1 KU","offer_id":53294150779245,"sku":"14541ES80","price":655.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":53294155530605,"sku":"14541ES92","price":5875.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/14541_7e366c4b-63a9-4072-b3a7-a00c6f851fab.webp?v=1778483011","url":"https:\/\/www.ebiohippo.com\/products\/rnase-hii-glycerol-free-2-u-mu-l-bhz20800036","provider":"BioHippo","version":"1.0","type":"link"}