{"product_id":"rnase-inhibitor-bhp15200310","title":"RNase Inhibitor","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme Inhibitors\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eRNase Inhibitor is a recombinant RNase inhibitor expressed in soluble form in Escherichia coli. It performs equivalently to the ribonuclease inhibitor purified from human placenta. It is a protein of approximately 51 kDa with an isoelectric point (pI) of 4.7. RNase Inhibitor can specifically bind RNase A, B, and C with a non-covalent bond to form a 1:1 complex to inactivate RNase, and has a broad spectrum of RNase inhibitory activity. The inhibitor is active in buffers of 0–0.5 M NaCl, pH 5–8, and has the highest activity at pH 7.8. It protects the integrity of mRNA and improves the efficiency of transcription and translation, while avoiding the possible effects of using organic compound inhibitors. The inhibitor is compatible with commonly used reverse transcriptases and DNA polymerases in RT-PCR and RT-qPCR. Unlike the human placental inhibitor, the recombinant protein lacks two cysteine residues and is therefore more resistant to oxidation, making it better suited to reactions that are sensitive to high DTT, such as qPCR.\u003c\/p\u003e\n\u003cp\u003eThe inhibitor is supplied as a single-component solution at 40 U\/µL in 20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 5 mM DTT and 50% glycerol. One activity unit is the amount of enzyme that inhibits 50% of the activity of 5 ng of RNase A. SDS-PAGE shows a single band at a molecular weight of 50 kDa.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e40 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUnit definition\u003c\/th\u003e\n\u003ctd\u003eAmount of enzyme that inhibits 50% of the activity of 5 ng of RNase A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003ctd\u003eRecombinant (E. coli)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e~51 kDa (SDS-PAGE shows a single band at ~50 kDa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsoelectric point\u003c\/th\u003e\n\u003ctd\u003epI 4.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eBinds RNase A, B and C non-covalently to form a 1:1 complex\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActive range\u003c\/th\u003e\n\u003ctd\u003epH 5–8 in buffers of 0–0.5 M NaCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal pH\u003c\/th\u003e\n\u003ctd\u003e7.0–8.0; highest activity at pH 7.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eComposition\u003c\/th\u003e\n\u003ctd\u003e20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 5 mM DTT, 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003eIsolation of polysomes\u003c\/li\u003e\n\u003cli\u003eIn vitro translation\u003c\/li\u003e\n\u003cli\u003eIn vitro cell-free system transcription\u003c\/li\u003e\n\u003cli\u003eIn vitro transcription with SP6 or T7 RNA polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C with a shelf life of one year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFoaming or strong agitation such as vortexing can cause deactivation.\u003c\/li\u003e\n\u003cli\u003eUse the inhibitor within its active range of pH 5–8 and 0–0.5 M NaCl.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ010\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHP15200310\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"5000 U","offer_id":53965434225005,"sku":"EQ010-5000U","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ010-a-1.jpg?v=1789416776","url":"https:\/\/www.ebiohippo.com\/products\/rnase-inhibitor-bhp15200310","provider":"BioHippo","version":"1.0","type":"link"}