{"product_id":"rnase-r-gmp-grade-20-u-mu-l-bhz20800038","title":"RNase R (GMP-grade, 20 U\/μL)","description":"\u003cp style=\"font-weight: 400;\"\u003eRibonuclease R (RNase R) from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e, is a magnesium-dependent 3′→5′ exoribonuclease that\u003cspan\u003e \u003c\/span\u003ecan digest all linear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. RNase R is used for enrichment of circRNAs in biological samples, identification of intronic lariat sequences, identification of exonic circRNAs, studying alternative splicing.\u003c\/p\u003e\n\u003carticle class=\"4ever-article\"\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003eRNase R must be used in combination with the 10× RNase R Reaction Buffer, GMP-grade (Cat. No. 14616ES)\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/article\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eFeatures  \u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eGuaranteed efficacy and quality with ISO 13485 certification.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eAvailable in bulk or tailored configurations.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eEffectively breaks down linear RNA, while preserving lariat loops and circular RNAs.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSelective digestion of linear RNAs enhances the isolation of circular RNAs for protein production or intronic cDNA library construction.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOffering GMP-Grade RNAse R\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eSpecificntions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eA recombinant \u003cem\u003eE. coli\u003c\/em\u003e strain carrying the RNAse R gene from \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl,100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, 1 mM DTT, 50% glycerol，pH7.5 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R serves as a fundamental component in circular RNA synthesis (circRNA). CircRNA holds promise for enhancing pharmaceutical stability and biostability. With their covalently closed structures, circRNAs withstand RNA exonucleases. [1,2] RNase R finds widespread application in various studies, including alternative splicing, intron cDNA production, and the isolation of splicing intermediates and lariats.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES25\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES72\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (GMP-grade, 20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003ePrepare the following reaction system in a sterile microcentrifuge tube\u003c\/p\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ecomponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10×RNase R Reaction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNA sample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1\u003cspan\u003e \u003c\/span\u003eμg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2-4 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eUp to 20\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIncubate at 37 ℃ for 10 - 30 minutes.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\n\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-1_600x600.jpg?v=1744210829\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 1: Total mRNA (C) can be digested by Yeasen RNAse R. Total mRNA input: 500ng. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-2_600x600.jpg?v=1744210830\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 2: CircRNA can not be digested by Yeasen RNAse R. CircRNA input: 500ng, RNAse R: 4U. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-3_1024x1024.jpg?v=1744210830\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 3: Performence of Yeasen RNAse R  (1U to 8U ) digestion on 1 μg of linear mRNA (C).\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe activity of RNase R requires 0.1-1.0 mM Mg\u003csup\u003e2+\u003c\/sup\u003e;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eWith the increase of RNA, the reaction time can be extended and the enzyme amount can be increased appropriately;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe EDTA content in RNA samples may affect the activity of RNase R.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eIncubate at 70 ℃ for 10 minutes can inactivate the enzyme.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150517101,"sku":"14615ES25","price":205.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294155596141,"sku":"14615ES72","price":1685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/14615-1_600x600_2b00ec45-e878-4f09-af12-17359ab82dd3.jpg?v=1778101060","url":"https:\/\/www.ebiohippo.com\/products\/rnase-r-gmp-grade-20-u-mu-l-bhz20800038","provider":"BioHippo","version":"1.0","type":"link"}