{"product_id":"rnf111-antibody-ring-finger-protein-111-bha17135411","title":"RNF111 Antibody \/ RING finger protein 111","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRNF111 Antibody \/ RING finger protein 111 is a anti-RNF111 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RNF111\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRNF111 antibody detects RING finger protein 111, an E3 ubiquitin-protein ligase that regulates DNA damage signaling, TGF-beta signaling, and transcriptional control. Also known as Arkadia, RNF111 facilitates ubiquitin-dependent degradation of transcriptional repressors to enhance signal transduction. The RNF111 antibody is used in studies of ubiquitin signaling, DNA repair, and cancer biology.\u003cbr\u003e\u003cbr\u003eRNF111 is encoded by the RNF111 gene on human chromosome 15q22.33. The protein is approximately 101 kilodaltons and contains a RING finger domain, two nuclear localization signals, and several coiled-coil regions. It acts as an E3 ligase that mediates ubiquitination of SMAD7 and SKI, thereby amplifying TGF-beta and BMP signaling cascades that control differentiation and tissue remodeling.\u003cbr\u003e\u003cbr\u003eThe RNF111 antibody identifies a 100-105 kilodalton nuclear protein by western blot. Immunofluorescence reveals punctate nuclear distribution consistent with its role in transcriptional regulation. RNF111 participates in DNA damage response through ubiquitination of SUMO-modified proteins at sites of double-strand breaks, promoting recruitment of repair factors. Loss or mutation of RNF111 results in defective DNA repair and impaired transcriptional activation of TGF-beta-responsive genes.\u003cbr\u003e\u003cbr\u003eRNF111 also modulates embryonic development, neural differentiation, and tumor suppression. Overexpression has been associated with hepatocellular carcinoma, whereas downregulation disrupts differentiation signaling in stem cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RNF111 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RNF111 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RNF111 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RNF111 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RNF111+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291085165,"sku":"FY12508","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f00e2ff9-fbc9-4400-8570-f5b5f5e65466.jpg?v=1782237007","url":"https:\/\/www.ebiohippo.com\/products\/rnf111-antibody-ring-finger-protein-111-bha17135411","provider":"BioHippo","version":"1.0","type":"link"}