{"product_id":"rnf115-antibody-ring-finger-protein-115-bha17135677","title":"RNF115 Antibody \/ Ring finger protein 115","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRNF115 Antibody \/ Ring finger protein 115 is a anti-RNF115 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R26 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RNF115\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30R26, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRNF115 antibody detects ring finger protein 115, encoded by the RNF115 gene. This protein is also known as breast cancer associated gene 2, BCA2, and Rabring7. RNF115 is an E3 ubiquitin protein ligase containing a RING finger domain that mediates transfer of ubiquitin from E2 conjugating enzymes to substrate proteins. It localizes to endosomes, lysosomes, and the plasma membrane, where it regulates receptor trafficking, immune signaling, and protein degradation. RNF115 expression has been detected in breast, lung, and immune tissues, highlighting its broad physiological roles.\u003cbr\u003e\u003cbr\u003eRNF115 antibody is widely applied in cancer research, immunology, and cell biology. Originally identified as BCA2 in breast cancer, RNF115 is implicated in tumor progression and resistance to therapy. As an E3 ligase, it regulates receptor endocytosis, innate immune signaling, and viral restriction pathways. By detecting RNF115, researchers can explore how ubiquitin mediated regulation impacts cancer and immunity.\u003cbr\u003e\u003cbr\u003eApplications for RNF115 antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies RNF115 in tumor and immune cell lysates, immunohistochemistry maps expression in cancer biopsies, and immunofluorescence reveals subcellular localization at endosomes. These techniques provide comprehensive coverage of RNF115 biology in experimental and clinical research.\u003cbr\u003e\u003cbr\u003eRNF115 interacts with Rab7 and other trafficking proteins to control endosomal sorting. It also modulates innate immune signaling by regulating proteins such as MAVS and STING. Viral proteins can target RNF115 to escape host defense, making it a focus in virology. In breast and other cancers, RNF115 overexpression correlates with proliferation, migration, and poor prognosis. By applying RNF115 antibody, scientists can link ubiquitin signaling to diverse disease processes.\u003cbr\u003e\u003cbr\u003eTherapeutic targeting of E3 ligases is an emerging strategy in drug discovery. RNF115 represents a potential target in cancer and infectious disease, where modulating ubiquitin transfer may alter signaling outcomes. The antibody provides a powerful tool for mechanistic studies and translational research exploring these therapeutic avenues.\u003cbr\u003e\u003cbr\u003eRNF115 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RNF115 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RNF115 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RNF115 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RNF115 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RNF115+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047299146093,"sku":"FY12774","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_98912efa-0143-414b-afa7-0967efe35c17.jpg?v=1772019341","url":"https:\/\/www.ebiohippo.com\/products\/rnf115-antibody-ring-finger-protein-115-bha17135677","provider":"BioHippo","version":"1.0","type":"link"}