{"product_id":"rnf25-antibody-ring-finger-protein-25-bha17135898","title":"RNF25 Antibody \/ Ring finger protein 25","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRNF25 Antibody \/ Ring finger protein 25 is a anti-RNF25 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RNF25\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRNF25 antibody detects Ring finger protein 25, an E3 ubiquitin ligase that participates in protein degradation, signaling regulation, and transcriptional control. The UniProt recommended name is Ring finger protein 25 (RNF25). This protein belongs to the RING-type E3 ligase family, which mediates the transfer of ubiquitin from E2 conjugating enzymes to substrate proteins, marking them for proteasomal degradation or signaling modification.\u003cbr\u003e\u003cbr\u003eFunctionally, RNF25 antibody identifies a 459-amino-acid cytoplasmic and nuclear protein containing a C3H2C3-type RING finger domain essential for ubiquitin ligase activity. RNF25 acts as a regulatory adapter that promotes the assembly of ubiquitin ligase complexes and modulates the stability of key signaling molecules. It interacts with components of the NF-kappaB signaling pathway, such as TRAF2 and RelA\/p65, thereby influencing inflammatory gene expression and apoptosis.\u003cbr\u003e\u003cbr\u003eThe RNF25 gene is located on chromosome 15q22.31 and encodes a protein expressed in multiple tissues, including brain, liver, and immune cells. It functions in the ubiquitin-proteasome system to regulate protein turnover and signaling dynamics. RNF25 has been shown to enhance NF-kappaB transcriptional activity by stabilizing the p65 subunit, amplifying responses to cytokines and stress stimuli. It may also participate in synaptic signaling and neuronal plasticity through regulation of protein degradation in neurons.\u003cbr\u003e\u003cbr\u003eBeyond its role in NF-kappaB signaling, RNF25 influences other cellular processes such as apoptosis, transcriptional repression, and immune modulation. It interacts with Smurf1 and other E3 ligases to fine-tune ubiquitin-dependent degradation networks. Dysregulation of RNF25 expression or activity has been linked to cancer progression, chronic inflammation, and neurodegenerative disease. Overexpression of RNF25 promotes survival pathways, while loss of function increases sensitivity to apoptotic stimuli.\u003cbr\u003e\u003cbr\u003eRNF25 antibody is widely used in research focused on protein degradation, signal transduction, and transcriptional regulation. It is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect RNF25 expression and localization. This antibody supports studies exploring the ubiquitin-proteasome system, NF-kappaB activation, and E3 ligase interactions. In disease models, RNF25 serves as a biomarker of altered ubiquitin signaling and inflammatory response.\u003cbr\u003e\u003cbr\u003eStructurally, RNF25 features a RING-type zinc-binding domain, a coiled-coil region for protein interactions, and nuclear localization motifs that enable shuttling between cytoplasm and nucleus. Post-translational regulation includes phosphorylation and auto-ubiquitination that modulate its ligase activity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RNF25 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RNF25 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RNF25 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RNF25 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RNF25+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309107565,"sku":"FY12996","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ba43d6ad-b279-4c75-9b65-50c5581b6318.jpg?v=1772019379","url":"https:\/\/www.ebiohippo.com\/products\/rnf25-antibody-ring-finger-protein-25-bha17135898","provider":"BioHippo","version":"1.0","type":"link"}