{"product_id":"rnf7-antibody-ring-finger-protein-7-bha17135782","title":"RNF7 Antibody \/ Ring finger protein 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRNF7 Antibody \/ Ring finger protein 7 is a anti-RNF7 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R91 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RNF7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30R91, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRNF7 antibody detects Ring finger protein 7, encoded by the RNF7 gene. Ring finger protein 7 is a small RING type E3 ubiquitin ligase that is an essential component of the SCF (SKP1 CUL1 F box) ubiquitin ligase complex. This complex targets key regulatory proteins for ubiquitin mediated degradation, ensuring proper cell cycle progression, signal transduction, and stress responses. RNF7 antibody allows researchers to study the regulation of protein turnover and the molecular machinery that controls cellular homeostasis through ubiquitination.\u003cbr\u003e\u003cbr\u003eRing finger protein 7 has multiple cellular roles, including redox regulation, neddylation, and tumor suppressor control. By facilitating ubiquitination, RNF7 ensures timely degradation of proteins such as p27 and IkappaB, which govern cell cycle checkpoints and NF kappa B signaling respectively. Studies using RNF7 antibody have shown that disruption of this E3 ligase leads to aberrant accumulation of these regulators, causing uncontrolled proliferation or impaired immune responses. RNF7 also interacts with antioxidant pathways by mediating degradation of pro oxidant proteins, supporting redox balance in the cell.\u003cbr\u003e\u003cbr\u003eAltered expression of Ring finger protein 7 has been implicated in cancer and degenerative diseases. Overexpression of RNF7 has been reported in several human cancers, including prostate, pancreatic, and lung carcinoma, where it may promote tumorigenesis by reducing levels of pro apoptotic proteins. Conversely, loss of function mutations impair ubiquitin ligase activity, leading to genomic instability. RNF7 antibody has been applied to studies that link this factor to chemotherapy resistance and cancer progression, making it an attractive target for drug discovery.\u003cbr\u003e\u003cbr\u003eRNF7 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting detects expression in cancer cell lines, while immunohistochemistry demonstrates its nuclear and cytoplasmic distribution in tissue sections. Immunoprecipitation with RNF7 antibody enables isolation of the SCF complex for functional studies. Together, these applications allow researchers to investigate its role in proteostasis, stress response, and tumor biology.\u003cbr\u003e\u003cbr\u003eBy providing validated RNF7 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RNF7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RNF7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RNF7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RNF7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RNF7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047304880493,"sku":"FY12880","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0e68a045-b10a-45cf-b147-9c0ec059d0ae.jpg?v=1772019355","url":"https:\/\/www.ebiohippo.com\/products\/rnf7-antibody-ring-finger-protein-7-bha17135782","provider":"BioHippo","version":"1.0","type":"link"}