{"product_id":"ro-51-bhb21300311","title":"Ro-51","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 2-[[4-amino-5-(5-iodo-4-methoxy-2-propan-2-ylphenoxy)pyrimidin-2-yl]amino]propane-1,3-diol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 1050670-85-3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C17H23IN4O4.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98% (HPLC)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 0.2 nM - 0.2 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Up to 100 mM in DMSO or Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e P2X3 receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Ro-51 is a highly potent and selective antagonist of P2X3 and P2X2\/3 receptors. It has been shown to inhibit rat homomeric P2X3 and human heteromeric P2X2\/3 receptors with pIC50 values of 8.7 and 8.3, respectively, whilst exhibiting no antagonistic activity at other P2X receptor family members at up to 10 µM1.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eRo-51 is a highly potent and selective antagonist of P2X3 and P2X2\/3 receptors. It inhibits rat homomeric P2X3 and human heteromeric P2X2\/3 and demonstrates IC50 values of 2 and 5 nM respectively1.Ro-51 has been shown to reduced hyperalgesia and mechanical allodynia in inflammatory and neuropathic pain models1,2. The compound is highly selective for P2X3 and P2X2\/3, showing no antagonistic activity at other P2X receptor family members1.P2X receptors are a family of ion channels gated by ATP. They are widely localized in cell types of almost every origin, including neuronal, muscular, epithelial and immune and have been shown to play a pivotal role in models of various pain conditions3.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249713602925,"sku":"R-195-10MG-1","price":235.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311575687533,"sku":"R-195-25MG-1","price":527.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311575720301,"sku":"R-195-5MG-1","price":168.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311575753069,"sku":"R-195-50MG-1","price":960.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/R-195_gr.gif?v=1778544449","url":"https:\/\/www.ebiohippo.com\/products\/ro-51-bhb21300311","provider":"BioHippo","version":"1.0","type":"link"}