{"product_id":"rock1-antibody-rho-associated-coiled-coil-containing-protein-kinase-1-bha17136234","title":"ROCK1 Antibody \/ Rho associated coiled coil containing protein kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eROCK1 Antibody \/ Rho associated coiled coil containing protein kinase 1 is a anti-ROCK1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32R84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ROCK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32R84, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eROCK1 antibody detects Rho associated coiled coil containing protein kinase 1, encoded by the ROCK1 gene. Rho associated coiled coil containing protein kinase 1 is a serine-threonine kinase that mediates actin cytoskeleton organization, cell adhesion, and motility downstream of Rho GTPases. ROCK1 antibody provides researchers with an important reagent to study signal transduction, cytoskeletal remodeling, and disease processes.\u003cbr\u003e\u003cbr\u003eRho associated coiled coil containing protein kinase 1 is activated by binding to the small GTPase RhoA. Research using ROCK1 antibody has shown that upon activation, the kinase phosphorylates substrates such as LIM kinase, myosin light chain phosphatase, and ERM proteins. These phosphorylation events regulate actin filament assembly, stress fiber formation, and focal adhesion dynamics. Through these activities, ROCK1 coordinates changes in cell shape and migration in response to extracellular signals.\u003cbr\u003e\u003cbr\u003eStudies with ROCK1 antibody have revealed that the kinase is essential for smooth muscle contraction, axon guidance, and angiogenesis. ROCK1 participates in vascular tone regulation by modulating smooth muscle contractility, while in the nervous system, it influences growth cone dynamics during axonal pathfinding. In endothelial cells, ROCK1 activity supports sprouting angiogenesis and vessel stability. These diverse roles emphasize its broad physiological importance.\u003cbr\u003e\u003cbr\u003eDysregulation of Rho associated coiled coil containing protein kinase 1 contributes to disease. Research using ROCK1 antibody has demonstrated that aberrant activation promotes cancer cell invasion and metastasis by remodeling the actin cytoskeleton. In cardiovascular disease, excessive ROCK1 activity contributes to vascular stiffness and hypertension. In neurological disorders, altered ROCK1 function affects axonal regeneration and contributes to neurodegeneration. These findings highlight ROCK1 as a therapeutic target in multiple pathological contexts.\u003cbr\u003e\u003cbr\u003ePharmacological inhibition of ROCK1 has been explored for therapeutic benefit. Studies with ROCK1 antibody have supported the development of selective inhibitors such as fasudil and ripasudil, which show efficacy in treating pulmonary hypertension, glaucoma, and other conditions involving abnormal ROCK signaling. This underscores the clinical relevance of monitoring ROCK1 expression and activity.\u003cbr\u003e\u003cbr\u003eROCK1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects kinase expression and post-translational modifications, immunohistochemistry demonstrates tissue distribution, and immunofluorescence shows cytoplasmic localization associated with actin fibers. These applications make ROCK1 antibody versatile in cell biology and disease research.\u003cbr\u003e\u003cbr\u003eBy providing validated ROCK1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ROCK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ROCK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ROCK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ROCK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ROCK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322509677,"sku":"FY13332","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c12f5db4-066d-4df1-b879-45ac714b7c18.jpg?v=1772019430","url":"https:\/\/www.ebiohippo.com\/products\/rock1-antibody-rho-associated-coiled-coil-containing-protein-kinase-1-bha17136234","provider":"BioHippo","version":"1.0","type":"link"}