{"product_id":"rpap3-antibody-rna-polymerase-ii-associated-protein-3-bha17135220","title":"RPAP3 Antibody \/ RNA polymerase II-associated protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPAP3 Antibody \/ RNA polymerase II-associated protein 3 is a anti-RPAP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPAP3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPAP3 antibody detects RNA polymerase II-associated protein 3, encoded by the RPAP3 gene on chromosome 12q24.31. RPAP3 antibody is commonly used in transcription, chaperone, and cancer research. RPAP3 is a component of the R2TP cochaperone complex, which cooperates with HSP90 and HSP70 to stabilize and assemble large macromolecular complexes. It is involved in the maturation of RNA polymerase II, snoRNPs, PIKKs, and other critical protein assemblies.\u003cbr\u003e\u003cbr\u003eStructurally, RPAP3 is a ~75 kDa protein with multiple tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. These domains allow RPAP3 to bridge HSP90 and PIH1D1, central elements of the R2TP complex. Its N-terminal domain anchors it to the cochaperone machinery, while its C-terminal region facilitates substrate recognition and client protein assembly.\u003cbr\u003e\u003cbr\u003eFunctionally, RPAP3 stabilizes RNA polymerase II subunits, enabling proper transcriptional initiation and elongation. It also regulates assembly of snoRNPs, which support ribosomal RNA modification, and PIKKs, which are central to DNA damage response, nutrient sensing, and stress signaling. Researchers use RPAP3 antibody to explore protein complex maturation, transcriptional regulation, and chaperone pathways.\u003cbr\u003e\u003cbr\u003eClinically, RPAP3 dysregulation has been linked to cancer progression. Because it stabilizes oncogenic PIKKs such as mTOR, ATR, and ATM, RPAP3 supports tumor growth and therapy resistance. Its expression patterns may serve as biomarkers for prognosis and treatment response. \u003cbr\u003e\u003cbr\u003eExperimentally, RPAP3 antibody is applied in western blotting to detect the ~75 kDa protein, in immunoprecipitation to study R2TP complex partners, and in immunofluorescence microscopy to visualize nuclear and cytoplasmic distribution. Chromatin immunoprecipitation using RPAP3 antibody helps examine transcriptional regulation via RNA polymerase II stabilization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPAP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPAP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPAP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPAP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPAP3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284498797,"sku":"FY12317","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e97c3d9c-a776-427d-9d0c-198e62eb2c91.jpg?v=1782236988","url":"https:\/\/www.ebiohippo.com\/products\/rpap3-antibody-rna-polymerase-ii-associated-protein-3-bha17135220","provider":"BioHippo","version":"1.0","type":"link"}