{"product_id":"rpl14-antibody-60s-ribosomal-protein-l14-bha17135416","title":"RPL14 Antibody \/ 60S ribosomal protein L14","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPL14 Antibody \/ 60S ribosomal protein L14 is a anti-RPL14 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPL14\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPL14 antibody detects 60S ribosomal protein L14, a structural component of the large ribosomal subunit involved in translation and ribosome assembly. RPL14 is one of approximately 80 ribosomal proteins that form the functional ribosome, catalyzing peptide bond formation and mRNA decoding. The RPL14 antibody is used in studies of ribosome biogenesis, translational control, and nucleolar organization.\u003cbr\u003e\u003cbr\u003eRPL14 is encoded by the RPL14 gene located on human chromosome 3p21.3. The protein is about 23 kilodaltons and localizes predominantly in the cytoplasm and nucleolus. RPL14 integrates into the 60S ribosomal subunit near the peptidyl transferase center, stabilizing interactions between rRNA and ribosomal proteins during translation. Through these roles, RPL14 ensures accuracy and efficiency of protein synthesis.\u003cbr\u003e\u003cbr\u003eThe RPL14 antibody detects a 23 kilodalton band on western blot and demonstrates both cytoplasmic and nucleolar staining by immunofluorescence. RPL14 expression correlates with proliferative activity, and its upregulation is often seen in rapidly dividing cells or tumors. Inhibition of RPL14 synthesis activates ribosomal stress pathways, leading to p53 stabilization and growth arrest. Mutations or pseudogene-derived transcripts of RPL14 may contribute to tumorigenesis or ribosomopathies characterized by anemia and developmental defects.\u003cbr\u003e\u003cbr\u003eBeyond translation, RPL14 participates in extra-ribosomal functions, including regulation of apoptosis and cell cycle progression. Under stress conditions, RPL14 can interact with transcription factors or signaling proteins, modulating gene expression independent of ribosome assembly. Proteomic studies have also detected RPL14 in mitochondrial ribosome fractions, suggesting roles in organelle-specific translation.\u003cbr\u003e\u003cbr\u003eBecause ribosomal proteins are frequently deregulated in cancer, RPL14 serves as a biomarker for tumor cell proliferation and ribosome biogenesis activity. Elevated expression has been observed in colorectal, breast, and ovarian cancers, correlating with poor prognosis. Conversely, knockdown of RPL14 reduces protein synthesis and inhibits tumor growth.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPL14 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPL14 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPL14 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPL14 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPL14+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291674989,"sku":"FY12513","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9f1f31bb-8da7-4f45-90d0-5e45a46a0a4f.jpg?v=1782237014","url":"https:\/\/www.ebiohippo.com\/products\/rpl14-antibody-60s-ribosomal-protein-l14-bha17135416","provider":"BioHippo","version":"1.0","type":"link"}