{"product_id":"rpl27-antibody-60s-ribosomal-protein-l27-bha17136172","title":"RPL27 Antibody \/ 60S ribosomal protein L27","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPL27 Antibody \/ 60S ribosomal protein L27 is a anti-RPL27 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPL27\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPL27 antibody detects 60S ribosomal protein L27, a structural component of the large ribosomal subunit that plays a vital role in protein synthesis. The UniProt recommended name is 60S ribosomal protein L27 (RPL27). As part of the ribosome's peptidyl transferase center, RPL27 contributes to rRNA stabilization, ribosome assembly, and elongation during translation.\u003cbr\u003e\u003cbr\u003eFunctionally, RPL27 antibody identifies a 136-amino-acid basic protein located in the cytoplasm and nucleus. RPL27 integrates into the 60S ribosomal subunit where it binds to 28S rRNA and helps maintain the structural framework necessary for catalytic activity during peptide bond formation. It interacts with ribosomal proteins such as RPL10 and RPL23, forming contacts that ensure ribosome stability and proper subunit joining. RPL27 also plays a role in ribosome biogenesis within the nucleolus, participating in pre-rRNA processing and export of mature ribosomal particles to the cytoplasm.\u003cbr\u003e\u003cbr\u003eThe RPL27 gene is located on chromosome 17q21.31 and is expressed ubiquitously in all actively proliferating cells. Expression levels correlate with translational demand, increasing during cell growth, tissue regeneration, and tumor progression. RPL27, like many ribosomal proteins, is co-transcribed with small nucleolar RNAs that regulate rRNA modification.\u003cbr\u003e\u003cbr\u003ePathologically, altered RPL27 expression has been associated with cancer and ribosomopathies. Overexpression promotes tumor cell proliferation by enhancing global translation and activating oncogenic pathways, while ribosomal stress due to RPL27 deficiency triggers p53 stabilization and apoptosis. RPL27 mutations may contribute to bone marrow failure syndromes and developmental disorders linked to impaired ribosome function. Research using RPL27 antibody supports studies in translation regulation, cancer metabolism, and ribosome assembly.\u003cbr\u003e\u003cbr\u003eRPL27 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ribosomal proteins. \u003cbr\u003e\u003cbr\u003eStructurally, 60S ribosomal protein L27 is composed of alpha-helical segments that interface with rRNA and neighboring ribosomal proteins, stabilizing the peptidyl transferase center. Its compact fold enables close packing within the ribosome's core. This antibody enables detailed examination of RPL27's role in ribosome assembly, translational fidelity, and cellular protein synthesis regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPL27 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPL27 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPL27 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPL27 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPL27+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320084845,"sku":"FY13270","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d7943e19-cd52-483d-9c2f-6e17f517287d.jpg?v=1782237085","url":"https:\/\/www.ebiohippo.com\/products\/rpl27-antibody-60s-ribosomal-protein-l27-bha17136172","provider":"BioHippo","version":"1.0","type":"link"}