{"product_id":"rplp0-antibody-biotin-conjugated-bha10505004","title":"RPLP0 Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-RPLP0 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. Designed to detect RPLP0 in Human, supporting researchers in epigenetics and nuclear signaling contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human 60S acidic ribosomal protein P0 protein (1-317AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A\/G compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin conjugate:\u003c\/strong\u003e Signal amplification via streptavidin\/avidin-coupled reporters. Block endogenous biotin in liver, kidney, or biotin-supplemented samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRPLP0\u003c\/strong\u003e (also known as 36B4, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E, Acidic ribosomal phosphoprotein P0, Arbp, L10E, LP0, MGC107165, MGC107166, MGC111226, MGC88175, OTTMUSP00000015585, P0, PRLP0, Ribosomal phosphoprotein, acidic, P0, Ribosomal protein P0, Ribosomal protein, large, P0, RLA0_HUMAN, rplP0, RPP0) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of RPLP0 biology relevant to epigenetics and nuclear signaling.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA quantifies soluble\/secreted target in biological fluids or cell culture supernatants.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Include IgG from Rabbit at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity\/isoforms:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53238708896109,"sku":"CSB-PA020336LD01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53238832595309,"sku":"CSB-PA020336LD01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA020336LA01HU-WB-2_66dd335b-353d-4579-9cc0-547dbc18f545.jpg?v=1782237625","url":"https:\/\/www.ebiohippo.com\/products\/rplp0-antibody-biotin-conjugated-bha10505004","provider":"BioHippo","version":"1.0","type":"link"}