{"product_id":"rpn1-antibody-ribophorin-i-bha17136156","title":"RPN1 Antibody \/ Ribophorin I","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPN1 Antibody \/ Ribophorin I is a anti-RPN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32R32 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32R32, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPN1 antibody detects Ribophorin I, encoded by the RPN1 gene. Ribophorin I is an integral membrane glycoprotein of the rough endoplasmic reticulum and a component of the oligosaccharyltransferase complex. This complex mediates the transfer of core oligosaccharides to nascent polypeptides, a key step in protein N linked glycosylation. RPN1 antibody provides researchers with an important reagent to study protein maturation, endoplasmic reticulum function, and secretory pathway biology.\u003cbr\u003e\u003cbr\u003eProtein glycosylation is a critical post translational modification that ensures proper folding, stability, and function of many secreted and membrane proteins. Research using RPN1 antibody has shown that Ribophorin I contributes to substrate recognition and complex stability within the oligosaccharyltransferase machinery. By supporting efficient glycosylation, RPN1 plays a vital role in quality control and trafficking of glycoproteins through the secretory pathway.\u003cbr\u003e\u003cbr\u003eStudies with RPN1 antibody have revealed that disruption of Ribophorin I function impairs N linked glycosylation, leading to misfolded proteins and activation of endoplasmic reticulum stress responses. Persistent dysfunction contributes to protein misfolding diseases and disorders of glycoprotein biosynthesis. This makes RPN1 a critical factor in maintaining protein homeostasis.\u003cbr\u003e\u003cbr\u003eIn cancer biology, RPN1 expression has been linked to tumor progression and therapeutic resistance. Research using RPN1 antibody has demonstrated that overexpression of Ribophorin I supports increased protein synthesis and folding capacity in tumor cells, enabling rapid proliferation and adaptation to stress. Elevated levels of RPN1 have been associated with poor prognosis in certain cancers, positioning it as a potential biomarker.\u003cbr\u003e\u003cbr\u003eRPN1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein expression and size, immunohistochemistry localizes RPN1 in rough endoplasmic reticulum rich tissues, and immunofluorescence reveals perinuclear reticular distribution. These applications make RPN1 antibody indispensable in research on protein folding and secretion.\u003cbr\u003e\u003cbr\u003eBy providing validated RPN1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047320412525,"sku":"FY13254","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c9968411-4817-4aa3-98bf-9f1b5b16ddee.jpg?v=1772019417","url":"https:\/\/www.ebiohippo.com\/products\/rpn1-antibody-ribophorin-i-bha17136156","provider":"BioHippo","version":"1.0","type":"link"}