{"product_id":"rps19-antibody-40s-ribosomal-protein-s19-bha17135302","title":"RPS19 Antibody \/ 40S ribosomal protein S19","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPS19 Antibody \/ 40S ribosomal protein S19 is a anti-RPS19 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPS19\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe RPS19 antibody targets 40S ribosomal protein S19, a structural component of the small ribosomal subunit encoded by the RPS19 gene. This protein is essential for ribosome biogenesis, rRNA maturation, and translation initiation. 40S ribosomal protein S19 interacts with ribosomal RNA and multiple assembly factors to promote 40S subunit stability and cytoplasmic export. The RPS19 antibody provides researchers with a high-specificity reagent to study ribosome assembly, protein synthesis, and ribosomopathies associated with defective ribosome production.\u003cbr\u003e\u003cbr\u003e40S ribosomal protein S19 is synthesized in the cytoplasm and imported into the nucleolus, where ribosome assembly begins. It binds pre-rRNA intermediates and contributes to proper folding and cleavage of the 18S rRNA precursor. The RPS19 antibody enables detection of this essential component within ribosomal particles, supporting studies of translational regulation and nucleolar dynamics. Its nucleolar localization reflects its participation in early 40S subunit maturation steps.\u003cbr\u003e\u003cbr\u003eMutations in RPS19 are the most common cause of Diamond-Blackfan anemia (DBA), a congenital erythroid aplasia characterized by defective red blood cell production. Loss of 40S ribosomal protein S19 impairs ribosome biogenesis, activates p53-mediated stress responses, and reduces erythroid progenitor survival. The RPS19 antibody supports investigation of these pathogenic mechanisms by enabling quantification of protein levels in patient-derived cells and experimental models. It also helps identify how ribosomal stress contributes to bone marrow failure and developmental abnormalities.\u003cbr\u003e\u003cbr\u003eIn addition to its ribosomal role, 40S ribosomal protein S19 has extra-ribosomal functions in apoptosis regulation, inflammation, and cell signaling. It can be released extracellularly where it acts as a chemotactic factor for macrophages. The RPS19 antibody provides a valuable means of analyzing these non-canonical functions, revealing how ribosomal proteins can serve signaling roles beyond translation. Dysregulation of RPS19 has also been observed in several cancers, linking ribosome synthesis with oncogenic growth control.\u003cbr\u003e\u003cbr\u003eThe RPS19 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry, yielding characteristic nucleolar and cytoplasmic staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPS19 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPS19 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPS19 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPS19 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPS19+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285842285,"sku":"FY12399","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_370b3740-9cdb-4c6e-8db9-8baebbc4e302.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/rps19-antibody-40s-ribosomal-protein-s19-bha17135302","provider":"BioHippo","version":"1.0","type":"link"}