{"product_id":"rps3a-antibody-40s-ribosomal-protein-s3a-bha17135444","title":"RPS3A Antibody \/ 40S ribosomal protein S3a","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPS3A Antibody \/ 40S ribosomal protein S3a is a anti-RPS3A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nucleolar.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPS3A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPS3A antibody detects 40S ribosomal protein S3a, a structural component of the small ribosomal subunit that plays essential roles in translation initiation and ribosome assembly. Beyond its function in protein synthesis, RPS3A has extra-ribosomal roles in apoptosis, cell proliferation, and stress response. The RPS3A antibody is used in studies of ribosome biogenesis, cancer, and cellular metabolism.\u003cbr\u003e\u003cbr\u003eRPS3A is encoded by the RPS3A gene on human chromosome 4q31.3. The protein is approximately 30 kilodaltons and associates with 18S rRNA in the 40S ribosomal subunit. It stabilizes rRNA folding and coordinates interactions between ribosomal proteins during translation initiation. RPS3A expression is ubiquitous but particularly elevated in rapidly dividing cells, reflecting its link to growth and biosynthetic capacity.\u003cbr\u003e\u003cbr\u003eThe RPS3A antibody detects a 30 kilodalton protein by western blot and shows cytoplasmic and nucleolar staining under immunofluorescence. RPS3A interacts with translation initiation factors and regulates mRNA selection and ribosome recruitment. It also influences mitochondrial protein synthesis and cellular energy metabolism.\u003cbr\u003e\u003cbr\u003eOutside the ribosome, RPS3A acts as a signaling modulator, interacting with viral proteins, apoptotic regulators, and stress response factors. Overexpression of RPS3A confers resistance to apoptosis and contributes to oncogenic transformation, while knockdown induces cell cycle arrest and autophagy. Elevated RPS3A expression has been detected in breast, liver, and lung cancers, correlating with poor prognosis and aggressive tumor behavior.\u003cbr\u003e\u003cbr\u003eDue to its dual role in translation and signaling, RPS3A serves as a valuable biomarker for cell proliferation and stress adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPS3A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPS3A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPS3A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPS3A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPS3A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292133741,"sku":"FY12541","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6382aa58-9da7-4e6f-8783-0d6e924f8d4c.jpg?v=1782237014","url":"https:\/\/www.ebiohippo.com\/products\/rps3a-antibody-40s-ribosomal-protein-s3a-bha17135444","provider":"BioHippo","version":"1.0","type":"link"}