{"product_id":"rps6ka3-antibody-ribosomal-protein-s6-kinase-alpha-3-bha17135421","title":"RPS6KA3 Antibody \/ Ribosomal protein S6 kinase alpha-3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPS6KA3 Antibody \/ Ribosomal protein S6 kinase alpha-3 is a anti-RPS6KA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPS6KA3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPS6KA3 antibody detects Ribosomal protein S6 kinase alpha-3, a serine\/threonine kinase that functions as a key effector in the MAPK signaling pathway. Also known as p90RSK2, this kinase transmits signals from extracellular stimuli to regulate cell growth, survival, and gene expression. The RPS6KA3 antibody is widely used in studies of neuronal development, signal transduction, and cancer biology.\u003cbr\u003e\u003cbr\u003eRPS6KA3 is encoded by the RPS6KA3 gene located on the X chromosome (Xp22.12). The protein is approximately 83 kilodaltons and contains two distinct kinase domains: an N-terminal kinase domain responsible for substrate phosphorylation and a C-terminal kinase domain that mediates activation through phosphorylation by ERK1\/2. This dual-kinase configuration enables fine-tuned regulation of RPS6KA3 activity in response to mitogenic and stress stimuli.\u003cbr\u003e\u003cbr\u003eThe RPS6KA3 antibody detects the full-length kinase as well as phosphorylated intermediates in western blot and immunofluorescence assays. RPS6KA3 phosphorylates transcription factors such as CREB, c-Fos, and histone H3, linking MAPK activation to transcriptional control. It also phosphorylates cytoplasmic substrates involved in mRNA translation and cytoskeletal rearrangement. The protein plays a central role in synaptic plasticity and long-term memory formation, highlighting its importance in neural signaling.\u003cbr\u003e\u003cbr\u003eMutations in RPS6KA3 cause Coffin-Lowry syndrome, an X-linked developmental disorder characterized by cognitive impairment and skeletal abnormalities. Disruption of RPS6KA3 signaling leads to defective ERK pathway regulation, impaired neuronal differentiation, and altered gene expression. Overactivation of RPS6KA3, on the other hand, contributes to oncogenic transformation through hyperactive MAPK signaling, promoting cell proliferation and resistance to apoptosis.\u003cbr\u003e\u003cbr\u003eBecause of its role in growth and survival pathways, RPS6KA3 serves as a potential therapeutic target in cancer and neurological disease. The RPS6KA3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPS6KA3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPS6KA3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPS6KA3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPS6KA3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPS6KA3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291052397,"sku":"FY12518","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_42b62f77-f87c-4ea9-953a-ca21af062d4a.jpg?v=1782237027","url":"https:\/\/www.ebiohippo.com\/products\/rps6ka3-antibody-ribosomal-protein-s6-kinase-alpha-3-bha17135421","provider":"BioHippo","version":"1.0","type":"link"}