{"product_id":"rps8-antibody-ribosomal-protein-s8-bha17135712","title":"RPS8 Antibody \/ Ribosomal protein S8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRPS8 Antibody \/ Ribosomal protein S8 is a anti-RPS8 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R72 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RPS8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30R72, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRPS8 antibody detects ribosomal protein S8, encoded by the RPS8 gene. This protein is also known as 40S ribosomal protein S8 and small subunit ribosomal protein S8. RPS8 is a component of the 40S ribosomal subunit, where it contributes to ribosome assembly and mRNA translation. As part of the ribosomal protein family, RPS8 interacts with ribosomal RNA and other proteins to ensure accurate decoding of mRNA into polypeptides. Because ribosomes are essential for protein synthesis, RPS8 is expressed in nearly all tissues and cell types.\u003cbr\u003e\u003cbr\u003eRPS8 antibody is widely applied in molecular biology, cancer research, and developmental biology. Ribosomal proteins serve not only structural roles but also regulate gene expression, cell growth, and stress responses. RPS8 has been implicated in ribosomopathies, tumorigenesis, and translational control. By detecting RPS8, researchers can study both canonical ribosome function and non ribosomal roles in cell regulation.\u003cbr\u003e\u003cbr\u003eApplications for RPS8 antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects RPS8 protein in cell lysates, immunohistochemistry maps expression in tissues, and immunofluorescence reveals nucleolar and cytoplasmic localization. These techniques provide robust approaches for studying ribosome biogenesis and translation.\u003cbr\u003e\u003cbr\u003eAltered ribosomal protein expression contributes to cancer, where increased protein synthesis supports uncontrolled proliferation. RPS8 overexpression has been observed in colorectal and lung cancers, where it correlates with tumor progression. Ribosomal stress caused by RPS8 deficiency activates p53 pathways, linking ribosome integrity to genome stability. By applying RPS8 antibody, scientists can explore how ribosomal proteins contribute to disease biology.\u003cbr\u003e\u003cbr\u003eBeyond cancer, RPS8 is studied in development and neurobiology. Ribosome assembly defects cause congenital disorders characterized by growth delay, anemia, and neurological symptoms. RPS8 participates in translational regulation during neuronal differentiation and synaptic activity. Detection with antibody based assays supports research into ribosomopathies and neurodevelopmental syndromes.\u003cbr\u003e\u003cbr\u003eRPS8 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RPS8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RPS8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RPS8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RPS8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RPS8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302848877,"sku":"FY12810","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c6be52d0-f1d8-4b13-8f8e-dc824c27139f.jpg?v=1772019353","url":"https:\/\/www.ebiohippo.com\/products\/rps8-antibody-ribosomal-protein-s8-bha17135712","provider":"BioHippo","version":"1.0","type":"link"}