{"product_id":"rre-reporter-lentivirus-bhv19400242","title":"RRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RAS-ERK pathway is a central signaling cascade in which growth factor activation of RAS GTPases drives the RAF-MEK-ERK kinase module, ultimately altering gene transcription that governs cell proliferation, differentiation, and survival. Activated ERK regulates transcription factors that bind the Ras-responsive element (RRE), including ETS family members such as ERG, ETV1, ETV4, and ETV5, as well as RREB-1. These factors mediate the gene expression changes associated with oncogenic transformation. Aberrant RAS-ERK signaling, whether through RAS mutation or ETS gene rearrangement, is a frequent driver of human cancers, making RRE-dependent transcription an informative readout of pathway activity and a target for inhibitor discovery.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RRE Reporter Lentivirus is a transcription-factor reporter system designed to detect transcription downstream of RAS-ERK signaling. The construct contains tandem repeats of the Ras-responsive element coupled to a minimal promoter, driving a fluorescent or luminescent reporter chosen from options including GFP, RFP, mCherry, BFP2, firefly luciferase, Gaussia luciferase, and dual configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe system is suited to monitoring RAS-ERK pathway activation, screening pathway inhibitors, and studying oncogenic signaling driven by ETS family transcription factors and RREB-1. Particles are purified by PEG precipitation and sucrose gradient centrifugation for effective transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RAS-ERK Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"RRE-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251624993133,"sku":"LTV-0135-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087428461,"sku":"LTV-0135-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087461229,"sku":"LTV-0135-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362087493997,"sku":"LTV-0135-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087526765,"sku":"LTV-0135-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087559533,"sku":"LTV-0135-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362087592301,"sku":"LTV-0135-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362087625069,"sku":"LTV-0135-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362087657837,"sku":"LTV-0135-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362087690605,"sku":"LTV-0135-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362087723373,"sku":"LTV-0135-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/rre.jpg?v=1776934141","url":"https:\/\/www.ebiohippo.com\/products\/rre-reporter-lentivirus-bhv19400242","provider":"BioHippo","version":"1.0","type":"link"}