{"product_id":"rs4-11-cell-bhc11101612","title":"RS4:11 cell","description":"The RS4:11 cell line is derived from a 32-year-old female patient with relapsed acute lymphoblastic leukemia (ALL) characterized by the t(4:11)(q21:q23) chromosomal translocation. This translocation results in the formation of the **KMT2A-AFF1 (formerly MLL-AF4)** fusion gene, which is a hallmark of this leukemia subtype. RS4:11 cells exhibit a biphenotypic profile, co-expressing both B-cell and monocytic markers, reflecting the mixed-lineage characteristics associated with this genetic rearrangement. The cell line is widely used as a model for understanding the biology of KMT2A-rearranged leukemias, which are associated with aggressive disease and poor prognosis.\n\nRS4:11 cells display features typical of pre-B lymphoblasts, including expression of markers such as CD19, HLA-DR, and terminal deoxynucleotidyl transferase (TdT), along with rearranged immunoglobulin heavy and light chain genes. Interestingly, upon treatment with differentiation-inducing agents like phorbol esters, RS4:11 cells adopt a monocyte-like phenotype, highlighting their lineage plasticity. This characteristic makes the cell line particularly valuable for studying the molecular drivers of differentiation and lineage commitment in leukemia.\n\nGenetically, the t(4:11) translocation disrupts the **KMT2A gene at 11q23**, fusing it with **AFF1 (AF4)** on 4q21, leading to a chimeric protein that aberrantly regulates gene expression, including Hox genes involved in hematopoietic development. RS4:11 cells have also been used to study secondary mutations, such as those in **FLT3**, which contribute to leukemogenesis and treatment resistance. The cell line serves as a robust preclinical model for testing targeted therapies, including inhibitors of the KMT2A-AFF1 interaction and agents aimed at associated signaling pathways.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101612\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950223126893,"sku":"305360","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/RS4_2011_20P1_2020x01_20281024_ch00_1920x1920_6694cb70-f733-49ec-a66a-02b1ee5052e5.jpg?v=1769069211","url":"https:\/\/www.ebiohippo.com\/products\/rs4-11-cell-bhc11101612","provider":"BioHippo","version":"1.0","type":"link"}