{"product_id":"rspo4-antibody-r-spondin-4-bha17135283","title":"RSPO4 Antibody \/ R-spondin-4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRSPO4 Antibody \/ R-spondin-4 is a anti-RSPO4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RSPO4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe RSPO4 antibody targets R-spondin-4, a secreted growth factor encoded by the RSPO4 gene that acts as a potent activator of Wnt\/beta-catenin signaling. R-spondin-4 belongs to the R-spondin family (RSPO1-4), which enhances Wnt receptor complex stability and signaling intensity through interactions with LGR4-6 receptors and the transmembrane E3 ubiquitin ligases RNF43 and ZNRF3. By amplifying Wnt responses, R-spondin-4 plays key roles in epithelial development, tissue repair, and organogenesis. The RSPO4 antibody enables precise detection of this signaling enhancer and supports research into developmental biology and regenerative medicine.\u003cbr\u003e\u003cbr\u003eR-spondin-4 is characterized by two cysteine-rich furin-like domains that mediate receptor binding and a thrombospondin type 1 (TSP1) domain that influences protein localization. Through these domains, R-spondin-4 stabilizes Frizzled and LRP5\/6 receptors on the cell surface, prolonging Wnt pathway activation. The RSPO4 antibody helps researchers examine this process by detecting protein expression in tissues where Wnt signaling drives growth and differentiation, including skin, limb, and nail matrix.\u003cbr\u003e\u003cbr\u003eMutations in RSPO4 cause anonychia congenita, a rare developmental disorder marked by complete or partial absence of nails. Such mutations impair Wnt pathway activation, underscoring R-spondin-4's essential role in ectodermal appendage development. The RSPO4 antibody is invaluable for exploring these mechanisms, allowing measurement of protein levels and localization in affected tissues. In experimental systems, recombinant R-spondin-4 is used to stimulate stem cell proliferation and differentiation, emphasizing its relevance in tissue engineering and regenerative therapies.\u003cbr\u003e\u003cbr\u003eBeyond development, R-spondin-4 has been implicated in tumorigenesis, particularly in cancers exhibiting aberrant Wnt signaling such as colorectal and gastric cancer. The RSPO4 antibody allows assessment of expression patterns in tumor samples and helps determine how altered RSPO4 activity contributes to cancer cell proliferation. In stem cell biology, this protein functions as a niche signal that maintains progenitor cell populations, making it a target of interest in organoid culture and tissue regeneration research.\u003cbr\u003e\u003cbr\u003eThe RSPO4 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields strong staining of secreted and extracellular matrix-associated R-spondin-4.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RSPO4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RSPO4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RSPO4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RSPO4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RSPO4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288168813,"sku":"FY12380","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c3334eed-408d-470f-bd68-4885a3bf7edc.jpg?v=1782237010","url":"https:\/\/www.ebiohippo.com\/products\/rspo4-antibody-r-spondin-4-bha17135283","provider":"BioHippo","version":"1.0","type":"link"}