{"product_id":"rtf2-antibody-replication-termination-factor-2-bha17135329","title":"RTF2 Antibody \/ Replication termination factor 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRTF2 Antibody \/ Replication termination factor 2 is a anti-RTF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RTF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe RTF2 antibody targets Replication termination factor 2, a nuclear protein encoded by the RTF2 gene that functions in DNA replication and replication-fork stability. Replication termination factor 2 is a component of the replication-stress response pathway that safeguards genome integrity by promoting proper fork processing and restart. The RTF2 antibody provides an essential tool for studying DNA replication, repair mechanisms, and cell-cycle regulation.\u003cbr\u003e\u003cbr\u003eReplication termination factor 2 associates with replication forks and interacts with the replisome to stabilize stalled replication structures. It is recruited to chromatin during S phase and removed after replication completion. The RTF2 antibody supports visualization of these dynamics, allowing researchers to monitor fork progression and resolution under genotoxic stress. RTF2 is critical for preventing aberrant fork degradation and double-strand break formation, thereby maintaining genomic stability.\u003cbr\u003e\u003cbr\u003eLoss of RTF2 leads to replication defects, increased DNA damage signaling, and activation of checkpoint kinases such as ATR and CHK1. The RTF2 antibody enables detection of expression changes in response to DNA damage or replication inhibitors, aiding studies into checkpoint control and genome maintenance. Its depletion causes hypersensitivity to replication-blocking agents and reduced cell viability, emphasizing its protective role during S phase.\u003cbr\u003e\u003cbr\u003eReplication termination factor 2 also participates in fork restart after hydroxyurea-induced stalling, coordinating reloading of replication machinery components. The RTF2 antibody allows mechanistic studies of this recovery process, revealing how RTF2 collaborates with helicases and nucleases to reestablish replication competence. Dysregulation of RTF2 expression has been implicated in cancer development and progression due to impaired replication fidelity.\u003cbr\u003e\u003cbr\u003eThe RTF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing characteristic nuclear staining consistent with its role in DNA metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RTF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RTF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RTF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RTF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RTF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287054701,"sku":"FY12426","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3d06471c-3f19-4cad-b21d-02e1d7ccb45d.jpg?v=1782237024","url":"https:\/\/www.ebiohippo.com\/products\/rtf2-antibody-replication-termination-factor-2-bha17135329","provider":"BioHippo","version":"1.0","type":"link"}