{"product_id":"rtkn2-antibody-rhotekin-2-bha17136135","title":"RTKN2 Antibody \/ Rhotekin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRTKN2 Antibody \/ Rhotekin 2 is a anti-RTKN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RTKN2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRTKN2 antibody detects Rhotekin 2, a Rho GTPase effector protein that regulates cytoskeletal dynamics, apoptosis, and immune cell signaling. The UniProt recommended name is Rhotekin 2 (RTKN2). This protein acts as a scaffold for RhoA-mediated signaling pathways that control actin organization, transcriptional activity, and cell survival.\u003cbr\u003e\u003cbr\u003eFunctionally, RTKN2 antibody identifies a 646-amino-acid cytoplasmic protein containing a Rho-binding domain (RBD) and ankyrin repeats that mediate protein-protein interactions. RTKN2 binds active GTP-bound RhoA to stabilize cytoskeletal structures and prevent apoptotic signaling. It also influences NF-kappaB activation and transcriptional regulation of immune and inflammatory genes. In T lymphocytes, RTKN2 promotes cell survival and proliferation by integrating RhoA and anti-apoptotic signaling pathways.\u003cbr\u003e\u003cbr\u003eThe RTKN2 gene is located on chromosome 10q21.1 and is highly expressed in lymphoid tissues, testis, and brain. Expression increases during T-cell activation and stress responses, indicating roles in immune signaling and cytoskeletal adaptation. RTKN2 contributes to both structural stability and signaling plasticity within dynamic cellular environments.\u003cbr\u003e\u003cbr\u003ePathologically, RTKN2 has been implicated in cancer and autoimmune disease. Overexpression enhances survival and proliferation of cancer cells through activation of NF-kappaB and suppression of apoptosis, whereas reduced RTKN2 expression may impair lymphocyte function and stress resistance. Research using RTKN2 antibody supports studies in Rho GTPase signaling, cytoskeletal dynamics, and immune cell regulation.\u003cbr\u003e\u003cbr\u003eRTKN2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect Rho effector proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Rhotekin 2 consists of a central RhoA-binding domain, ankyrin repeats, and multiple phosphorylation sites that control its localization and interaction with downstream effectors. Its structure enables binding to GTP-bound RhoA and integration with cell survival pathways. This antibody facilitates analysis of RTKN2's role in Rho-dependent signaling and immune cell function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RTKN2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RTKN2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RTKN2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RTKN2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RTKN2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320117613,"sku":"FY13233","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_caafc392-0856-422e-a30b-b7e064267980.jpg?v=1782237092","url":"https:\/\/www.ebiohippo.com\/products\/rtkn2-antibody-rhotekin-2-bha17136135","provider":"BioHippo","version":"1.0","type":"link"}