{"product_id":"rtraf-antibody-hcle-bha17135474","title":"RTRAF Antibody \/ hCLE","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRTRAF Antibody \/ hCLE is a anti-RTRAF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RTRAF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRTRAF antibody detects RNA transcription, translation, and transport factor, a multifunctional RNA-binding protein involved in mRNA export, transcriptional regulation, and translation initiation. RTRAF interacts with transcription machinery and translation factors, serving as a molecular bridge that coordinates gene expression at multiple levels. The RTRAF antibody is used in RNA biology, gene expression, and epigenetic studies to investigate transcriptional and post-transcriptional regulation.\u003cbr\u003e\u003cbr\u003eRTRAF is encoded by the RTRAF gene located on human chromosome 17q23.3. The protein is approximately 375 amino acids long and localizes to both the nucleus and cytoplasm, reflecting its diverse functions. It forms complexes with RNA polymerase II, transcription elongation factors, and RNA helicases, linking transcription with RNA processing and export. RTRAF also associates with translation initiation factor eIF3, regulating mRNA translation efficiency.\u003cbr\u003e\u003cbr\u003eThe RTRAF antibody detects a 43 kilodalton band by western blot and demonstrates both nuclear speckle and cytoplasmic punctate staining in immunofluorescence assays. RTRAF participates in gene expression control by influencing promoter clearance, mRNA splicing, and ribosome loading. Its loss disrupts RNA metabolism, leading to reduced protein synthesis and altered stress granule dynamics.\u003cbr\u003e\u003cbr\u003eFunctionally, RTRAF has been linked to viral replication, as it enhances viral RNA translation and replication complex assembly in infected cells. In cancer, RTRAF expression correlates with increased proliferation and tumor aggressiveness due to its role in sustaining translation of growth-related mRNAs. It also contributes to chromatin organization by interacting with histone modifiers and transcriptional regulators.\u003cbr\u003e\u003cbr\u003eThrough its integration of transcription, RNA transport, and translation, RTRAF acts as a key coordinator of gene expression homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RTRAF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RTRAF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RTRAF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RTRAF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RTRAF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292756333,"sku":"FY12571","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6984b6ff-c973-4842-b69f-ec0c05734a88.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/rtraf-antibody-hcle-bha17135474","provider":"BioHippo","version":"1.0","type":"link"}