{"product_id":"runx1t1-antibody-bha10556444","title":"RUNX1T1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-RUNX1T1 antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect RUNX1T1 protein in Human, Mouse and supports researchers working in epigenetics and nuclear signaling contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthetic peptide of Human RUNX1T1 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRUNX1T1\u003c\/strong\u003e (also referred to as Acute myelogenous leukemia 1 translocation 1 cyclin D related, Acute myelogenous leukemia 1 translocation 1 protein, AML1 MTG8, AML1T1, CBFA2T1, CDR, Core binding factor runt domain alpha subunit 2 translocated to 1, Core binding factor runt domain alpha subunit 2 translocated to 1 cyclin D related, Cyclin D related, Cyclin D related protein, Cyclin-D-related protein, Eight twenty one protein, ETO, ETO protein, MGC2796, MTG 8, MTG 8b, MTG8, MTG8 protein, MTG8_HUMAN, MTG8b, Myeloid translocation gene on 8q22, Protein CBFA2T1, Protein ETO, Protein MTG 8, Protein MTG8, Runt related transcription factor 1 translocated to 1 (cyclin D related), Runt related transcription factor 1 translocated to 1, Runt related transcription factor 1 translocated to 1 cyclin D related, RUNX1 translocation partner 1, RUNX1T1, Zinc finger MYND domain containing protein 2, Zinc finger MYND domain-containing protein 2, ZMYND 2, ZMYND2) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RUNX1T1 biology in epigenetics and nuclear signaling.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human, Mouse. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 uL","offer_id":53247502680429,"sku":"CSB-PA066534-50UL","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53247593677165,"sku":"CSB-PA066534-100UL","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA066534-P.jpg?v=1776788164","url":"https:\/\/www.ebiohippo.com\/products\/runx1t1-antibody-bha10556444","provider":"BioHippo","version":"1.0","type":"link"}