{"product_id":"rxre-dr1-reporter-lentivirus-bhv19400043","title":"RXRE\/DR1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe retinoid X receptor (RXR) is a nuclear hormone receptor that acts as a master regulator of transcription by binding specific DNA sequences in the promoters of target genes. RXR recognizes direct-repeat elements such as DR1, and its activity depends on dimerization state: RXR\/RXR homodimers respond to 9-cis retinoic acid, whereas RXR\/RAR heterodimers can repress transcription through the same element. As an obligate partner for many other nuclear receptors, RXR integrates retinoid, lipid, and hormonal signals. Retinoic acid signaling through RXR governs cell differentiation, proliferation, and development, and dysregulation of this pathway is implicated in cancer and metabolic disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RXRE\/DR1 Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent (GFP or RFP) or luminescent (firefly luciferase) readout of RXR transcriptional activity in mammalian cells. The reporter contains tandem repeats of the consensus DR1 DNA-binding element, which is activated by RXR\/RXR homodimers and suppressed by RXR\/RAR heterodimers in response to 9-cis retinoic acid. It is used to study retinoic acid signaling and to quantify RXR-mediated transcription. All versions include a drug-selection marker (puromycin or blasticidin) for straightforward establishment of stable cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the RXR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251623780717,"sku":"LTV-0047-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896898413,"sku":"LTV-0047-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896931181,"sku":"LTV-0047-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896963949,"sku":"LTV-0047-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896996717,"sku":"LTV-0047-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310897029485,"sku":"LTV-0047-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897062253,"sku":"LTV-0047-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0047-RXRE-TAG-Puro-Figure-1.jpg?v=1776934400","url":"https:\/\/www.ebiohippo.com\/products\/rxre-dr1-reporter-lentivirus-bhv19400043","provider":"BioHippo","version":"1.0","type":"link"}