{"product_id":"ryanodine-receptor-1-stable-hek293-cell-line-bhc10901434","title":"Ryanodine Receptor 1 Stable HEK293 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eRyanodine receptors (RyR) are intracellular calcium channels located in the sarcoplasmic reticulum and the endoplasmic reticulum, and they mediate the release of calcium ions during muscle contractions. RyR are structurally and functionally similar to the inositol 1,4,5-triphosphate receptors (IP3R), which is responsible in initiating intracellular calcium signaling pathways.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Ryanodine Receptor 1 Stable HEK293 Cell Line is supplied as an engineered cell line derived from Human kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 15% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 100 µg\/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in kidney biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eMutations to the receptor have been observed in numerous diseases states. The Ryanodine Receptor 1 Stable HEK293 Cell Line stably expresses functional rabbit RyR1 calcium release channels, thus providing a valuable tool for pharmacological investigations in the function and mechanisms of calcium release channels activity, and in depth biophysical studies of the RyR1 receptor. Western blot and immunofluorescence analysis was used to confirm the expression of rabbit RyR1. Donor\/background information provided for this product: Embryo.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 15% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 100 µg\/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30,000 - 60,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180526625133,"sku":"T3037","price":525.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1JWnNCog0KKCB8fm8h9bgtc8Lbh23lU3tJq1h1FA.png?v=1774957819","url":"https:\/\/www.ebiohippo.com\/products\/ryanodine-receptor-1-stable-hek293-cell-line-bhc10901434","provider":"BioHippo","version":"1.0","type":"link"}