{"product_id":"ryk-antibody-tyrosine-protein-kinase-ryk-bha17135906","title":"RYK Antibody \/ Tyrosine-protein kinase RYK","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRYK Antibody \/ Tyrosine-protein kinase RYK is a anti-RYK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RYK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRYK antibody detects Tyrosine-protein kinase RYK, an atypical receptor tyrosine kinase that mediates Wnt signaling during neural development and tissue morphogenesis. The UniProt recommended name is Tyrosine-protein kinase RYK (RYK). Unlike conventional kinases, RYK possesses a catalytically inactive kinase domain but functions as a receptor and co-receptor for Wnt family ligands, influencing cellular polarity and differentiation.\u003cbr\u003e\u003cbr\u003eFunctionally, RYK antibody identifies a 604-amino-acid single-pass transmembrane receptor that binds Wnt proteins through its extracellular WIF (Wnt inhibitory factor) domain. Upon ligand binding, RYK cooperates with Frizzled receptors to activate non-canonical Wnt signaling pathways such as planar cell polarity and calcium flux regulation. This signaling guides axon pathfinding, neuronal connectivity, and tissue patterning during embryogenesis.\u003cbr\u003e\u003cbr\u003eThe RYK gene is located on chromosome 3q22.1 and encodes a receptor expressed in developing brain, spinal cord, and epithelial tissues. Although structurally related to receptor tyrosine kinases, its kinase domain lacks catalytic residues, functioning instead as a scaffolding site for adaptor proteins and kinases that propagate downstream signals. RYK influences Wnt-dependent activation of Dishevelled, JNK, and Rho family GTPases, affecting cytoskeletal organization and migration.\u003cbr\u003e\u003cbr\u003eIn the nervous system, RYK is essential for axonal guidance, dendritic growth, and synaptic remodeling. Loss of RYK function disrupts neuronal wiring and corticospinal tract formation. In epithelial tissues, RYK contributes to planar cell polarity and branching morphogenesis. Aberrant RYK signaling has been associated with cancer, fibrosis, and neurodevelopmental disorders. Overexpression of RYK in tumors promotes invasive growth by modulating cell adhesion and Wnt-dependent transcriptional programs.\u003cbr\u003e\u003cbr\u003eRYK antibody is widely used in developmental biology, neurobiology, and signaling research. It is suitable for immunoblotting, immunofluorescence, and receptor binding studies to detect RYK expression and localization at the plasma membrane. In neuroscience, this antibody supports studies of Wnt receptor composition and axon guidance. In cancer research, RYK detection helps characterize Wnt-driven signaling and invasive phenotypes.\u003cbr\u003e\u003cbr\u003eStructurally, RYK features an extracellular WIF domain that binds Wnt ligands, a single transmembrane helix, and an intracellular pseudokinase domain that serves as a docking platform for signaling complexes. Its signaling capacity depends on proteolytic cleavage and release of the intracellular domain, which can translocate to the nucleus and modulate gene transcription.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RYK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RYK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RYK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RYK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RYK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310025069,"sku":"FY13004","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_651ae7e2-27a1-4abb-b675-b7b8f9f3c6a7.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/ryk-antibody-tyrosine-protein-kinase-ryk-bha17135906","provider":"BioHippo","version":"1.0","type":"link"}