{"product_id":"s-pneumoniae-dna-primase-assay-kit-bht16300059","title":"S. pneumoniae DNA Primase Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDrug Target Assay Kit\u003c\/span\u003e\u003cp\u003eAt the DNA replication fork, DNA helicase (DnaB) opens double-stranded DNA and bacterial DNA primase (DnaG) builds the RNA primers. Helicases greatly boost primase activity in gram-negative bacteria such as E. coli and H. influenzae, but not in gram-positive S. aureus and S. pneumoniae.\u003c\/p\u003e\n\u003ch2\u003eKit vs Kit Plus\u003c\/h2\u003e\n\u003cp\u003eThe only difference is the enzyme: PGA100K supplies the assay reagents without enzyme, while PGA100KE adds 45 µl of 100 x S. pneumoniae primase. Both cover 100 assays in a 96-well plate or 200 assays in a 384-well plate.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eKit (enzyme not included), 100 assays (PGA100K)\u003c\/th\u003e\n\u003ctd\u003e100 assays (96-well) or 200 assays (384-well)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (enzyme included), 100 assays (PGA100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays (96-well) or 200 assays (384-well)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eKit (enzyme not included), 100 assays (PGA100K)\u003c\/th\u003e\n\u003cth\u003eKit Plus (enzyme included), 100 assays (PGA100KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Assay buffer\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x DNA template\u003c\/td\u003e\n\u003ctd\u003e45 µl\u003c\/td\u003e\n\u003ctd\u003e45 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x NTP mix\u003c\/td\u003e\n\u003ctd\u003e45 µl\u003c\/td\u003e\n\u003ctd\u003e45 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Fluorescence dye\u003c\/td\u003e\n\u003ctd\u003e850 µl\u003c\/td\u003e\n\u003ctd\u003e850 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x S. pneumoniae primase\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003ctd\u003e45 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eAssay solution: 10 mM MES pH 6.5, 5 mM magnesium acetate, 12.5 mM ammonium chloride, 0.5 mM DTT, 5 mM calcium chloride, 0.003% Brij-35, 100 nM DNA, 0.5 mM NTPs, 100 nM enzyme.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe assay measures the RNA primers made by DNA primase from a DNA template and NTPs. After the reaction a fluorescence dye is added and the signal is read at 535 nm (excitation 485 nm).\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eS. pneumoniae DNA primase activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eDNA Primase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eS. pneumoniae\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eEnzyme Activity Assay; Inhibitor Screening Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eEx 485 nm \/ Em 535 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e96-well or 384-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInstrument Required\u003c\/th\u003e\n\u003ctd\u003eFluorescence microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003eAbout 65 min (60 min reaction + 5 min detection)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReference\u003c\/th\u003e\n\u003ctd\u003eLacriola CJ et al., The Journal of Antibiotics 70, 918-920 (2017)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 assays (96-well) or 200 assays (384-well)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's protocol describes the procedure and reaction volumes.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eS. pneumoniae DNA Primase Assay (PDF, see Documents): Covers catalog # PGA100K, PGA100KE.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The S. pneumoniae DNA Primase Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (enzyme not included)","offer_id":67794878103917,"sku":"PGA100K","price":494.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (enzyme included)","offer_id":67794883019117,"sku":"PGA100KE","price":829.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EGA100KE_copy_2842fdd4-2146-4419-b685-badb39aed74a.jpg?v=1792791968","url":"https:\/\/www.ebiohippo.com\/products\/s-pneumoniae-dna-primase-assay-kit-bht16300059","provider":"BioHippo","version":"1.0","type":"link"}