{"product_id":"s100a10-antibody-bha17102654","title":"S100A10 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eS100A10 antibody supplied as a purified reagent for WB, ICC\/IF, IP in Human, Mouse, Rat samples. This product is a monoclonal (mouse origin) antibody (host: Mouse; isotype: Mouse lgG2a) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Monoclonal (mouse origin); host Mouse; isotype Mouse lgG2a; clone 6F4-E6-D5-C10.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein G affinity.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human, Mouse, Rat.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, ICC\/IF, IP.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A human recombinant partial protein was used as the immunogen for this S100A10 antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eS100A10 is the intended antigen for this primary antibody. Reported biological context includes: Because S100A10 induces the dimerization of ANXA2\/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003ePost-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.\u003c\/li\u003e   \u003cli\u003eSignal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.\u003c\/li\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eImmunocytochemistry \/ immunofluorescence (ICC\/IF): assess subcellular distribution and stimulus-dependent relocalization; co-localization analysis can support pathway or organelle hypotheses.\u003c\/li\u003e   \u003cli\u003eImmunoprecipitation (IP): enrich native target and associated complexes for downstream analysis (e.g., immunoblotting or mass spectrometry) and interaction mapping.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P08207) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P08207\/entry - NCBI Gene search (S100A10) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=S100A10 - Ensembl search (S100A10) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=S100A10 - PubMed search (S100A10) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=S100A10 - Reactome pathway search (S100A10) — Reactome — https:\/\/reactome.org\/content\/query?q=S100A10 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In PBS with 50% glycerol and 0.09% sodium azide \/ 0.1 ml","offer_id":53043212386669,"sku":"F54052-0.1ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f0a04cc0-992e-4204-9761-84ceceb99602.jpg?v=1771934129","url":"https:\/\/www.ebiohippo.com\/products\/s100a10-antibody-bha17102654","provider":"BioHippo","version":"1.0","type":"link"}