{"product_id":"sall2-antibody-spalt-like-transcription-factor-2-bha17135370","title":"SALL2 Antibody \/ Spalt-like transcription factor 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSALL2 Antibody \/ Spalt-like transcription factor 2 is a anti-SALL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SALL2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSALL2 antibody recognizes Spalt-like transcription factor 2, a zinc finger transcriptional regulator involved in embryonic development, stem cell maintenance, and tumor suppression. SALL2 belongs to the spalt-like family of transcription factors (SALL1-4), characterized by multiple C2H2-type zinc finger domains that bind DNA and regulate transcription of developmental and cell-cycle-related genes. The SALL2 antibody is commonly used in studies of neurogenesis, eye development, and cancer biology, where SALL2 functions as both a transcriptional activator and repressor depending on the cellular context.\u003cbr\u003e\u003cbr\u003eSALL2 is encoded by the SALL2 gene located on human chromosome 14q11.2. The protein localizes primarily to the nucleus and regulates genes controlling cell proliferation, apoptosis, and differentiation. During development, SALL2 contributes to optic nerve formation, limb morphogenesis, and neural tube closure. It interacts with other transcription factors such as p53, WT1, and SP1, integrating growth factor signaling with chromatin remodeling. Its expression is regulated by developmental cues and stress responses, suggesting broad involvement in maintaining genomic stability and tissue integrity.\u003cbr\u003e\u003cbr\u003eThe SALL2 antibody detects multiple isoforms ranging from 100-150 kDa depending on splicing and post-translational modifications. Western blot analyses demonstrate nuclear enrichment, while immunohistochemistry reveals SALL2 expression in neural tissues, retina, and developing mesenchyme. Functionally, SALL2 acts as a tumor suppressor by promoting p21 expression and inhibiting uncontrolled cell proliferation. Loss or downregulation of SALL2 has been associated with glioblastoma, ovarian carcinoma, and other cancers. Conversely, aberrant expression can disrupt differentiation programs and promote oncogenic transformation under certain signaling conditions.\u003cbr\u003e\u003cbr\u003eResearch has also revealed SALL2's role in stem cell pluripotency and differentiation. It interacts with key developmental regulators, including OCT4 and SOX2, and influences chromatin accessibility at developmental gene loci. In neural systems, SALL2 supports axon guidance and neuronal survival, linking it to neurological disorders when dysregulated.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SALL2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SALL2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SALL2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SALL2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SALL2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289479533,"sku":"FY12467","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_78f7b145-0ab5-415a-ac0b-e9ab74be773f.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/sall2-antibody-spalt-like-transcription-factor-2-bha17135370","provider":"BioHippo","version":"1.0","type":"link"}