{"product_id":"sb100x-bhn20152548","title":"SB100X","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003eSB100X\u003c\/strong\u003e, a transposase construct supplied for gene editing and genome engineering applications. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eTransposases are enzymes that catalyse the cut-and-paste (Class II) or copy-and-paste transposition of discrete DNA elements (transposons) into host genomes in a sequence-specific manner. Hyperactive Sleeping Beauty transposase (hyPBase) and Sleeping Beauty 100x (SB100X) are engineered variants of the \u003cem\u003eSalmo salar\u003c\/em\u003e Tc1\/mariner-family transposon system with substantially enhanced integration activity relative to the wild-type enzyme. Co-delivery of transposase mRNA with a donor plasmid carrying the transposon cargo offers a non-viral route to stable genomic integration, commonly employed in CAR T-cell manufacturing, gene therapy research, and stable cell line generation where viral vector capacity or safety restrictions limit alternative approaches.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eNon-viral CAR T manufacturing: Sleeping Beauty transposon systems with hyperactive transposase mRNA are in clinical-stage evaluation for cost-effective, GMP-compatible stable CAR integration into T cells.\u003c\/li\u003e\n  \u003cli\u003eStable reporter cell generation: Transposase mRNA + transposon plasmid co-delivery generates stable fluorescent or selection-marked cell lines without lentiviral handling constraints.\u003c\/li\u003e\n  \u003cli\u003eHigh-throughput genetic screen libraries: piggyBac (hyPBase) systems enable reversible, scarless transgene removal after selection due to transposon excision, supporting iterative genetic library strategies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eStable transgene integration — co-delivery of transposase mRNA with transposon donor plasmid for non-viral genomic integration of therapeutic or reporter constructs.\u003c\/li\u003e\n  \u003cli\u003eCAR T-cell manufacturing research — SB100X or hyPBase transposase mRNA in T-cell engineering studies for stable CAR expression without lentiviral production.\u003c\/li\u003e\n  \u003cli\u003eSelection cassette integration — stable antibiotic resistance marker insertion followed by transposase-mediated excision for scarless genetic modification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eTransposase mRNA activity requires co-delivery of a compatible transposon plasmid carrying ITR sequences flanking the cargo; confirm ITR compatibility with the specific transposase variant used (SB- or piggyBac-specific ITRs are not interchangeable).\u003c\/li\u003e\n  \u003cli\u003eIntegration site distribution is semi-random for both SB and hyPBase systems; for applications requiring site-specific integration, confirm acceptable genomic safety profiles by integration site analysis.\u003c\/li\u003e\n  \u003cli\u003eStable cell selection (antibiotic or FACS) should begin 48–72 h post-transfection; delayed selection may reduce clonal yield from incomplete transposition events.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239632855405,"sku":"IR0103002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239648158061,"sku":"IR0103010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239648190829,"sku":"IR0103020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239648223597,"sku":"IR0103050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239648256365,"sku":"IR0103100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239648289133,"sku":"IR0103500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_5c4b9e0e-fbcb-431b-826b-b736694d582b.png?v=1776677727","url":"https:\/\/www.ebiohippo.com\/products\/sb100x-bhn20152548","provider":"BioHippo","version":"1.0","type":"link"}