{"product_id":"scaf11-antibody-sr-related-ctd-associated-factor-11-sfrs2ip-bha17136171","title":"SCAF11 Antibody \/ SR-related CTD-associated factor 11 \/ SFRS2IP","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSCAF11 Antibody \/ SR-related CTD-associated factor 11 \/ SFRS2IP is a anti-SCAF11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SCAF11\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSCAF11 antibody detects SR-related CTD-associated factor 11, a nuclear protein that couples RNA processing to transcription by RNA polymerase II. The UniProt recommended name is SR-related CTD-associated factor 11 (SCAF11). Also known as SFRS2-interacting protein, SCAF11 belongs to a family of large SR-related proteins that integrate transcriptional elongation with pre-mRNA splicing and RNA surveillance pathways.\u003cbr\u003e\u003cbr\u003eFunctionally, SCAF11 antibody identifies a 1,450-amino-acid nuclear protein containing RNA recognition motifs (RRMs) and arginine\/serine-rich (RS) domains characteristic of SR proteins. SCAF11 binds to the C-terminal domain (CTD) of RNA polymerase II, recognizing its phosphorylation state and linking elongating polymerases to RNA splicing machinery. It interacts with splicing factors such as SRSF2 and SF3B1 and participates in the coordination of cotranscriptional mRNA processing.\u003cbr\u003e\u003cbr\u003eThe SCAF11 gene is located on chromosome 12q24.33 and is ubiquitously expressed, with higher levels in actively proliferating and transcriptionally active cells. Expression peaks during S-phase and in tissues with elevated RNA synthesis such as liver and brain. SCAF11 plays a dual role as a structural component of the transcriptional complex and a modulator of RNA maturation efficiency.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of SCAF11 has been linked to aberrant RNA processing and oncogenic transformation. Overexpression contributes to cancer progression by promoting transcriptional elongation of growth-associated genes, while knockdown disrupts RNA stability and gene expression homeostasis. SCAF11 has also been implicated in neurodegenerative conditions where transcriptional stress affects mRNA splicing fidelity. Research using SCAF11 antibody supports studies in transcriptional regulation, RNA processing, and chromatin biology.\u003cbr\u003e\u003cbr\u003eSCAF11 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcription-associated RNA-binding proteins. \u003cbr\u003e\u003cbr\u003eStructurally, SR-related CTD-associated factor 11 contains multiple RRMs for RNA binding and long RS-rich regions that mediate protein-protein interactions with splicing and elongation factors. Its CTD-binding domain recognizes phosphorylated Ser2 residues on RNA polymerase II, positioning SCAF11 at sites of active transcription. This antibody facilitates investigation of SCAF11's role in coordinating transcription, RNA processing, and post-transcriptional regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SCAF11 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SCAF11 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SCAF11 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SCAF11 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SCAF11+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321067885,"sku":"FY13269","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fadca8f5-8d25-4ce6-91ab-db006a319322.jpg?v=1772019462","url":"https:\/\/www.ebiohippo.com\/products\/scaf11-antibody-sr-related-ctd-associated-factor-11-sfrs2ip-bha17136171","provider":"BioHippo","version":"1.0","type":"link"}