{"product_id":"scap-antibody-srebp-cleavage-activating-protein-bha17135264","title":"SCAP Antibody \/ SREBP cleavage-activating protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSCAP Antibody \/ SREBP cleavage-activating protein is a anti-SCAP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SCAP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SCAP antibody targets SREBP cleavage-activating protein, a key membrane-bound regulator of cholesterol and lipid homeostasis encoded by the SCAP gene. This protein acts as a sterol sensor that governs the trafficking and activation of sterol regulatory element-binding proteins (SREBPs), transcription factors that control lipid biosynthesis. SREBP cleavage-activating protein monitors cellular sterol levels and facilitates the transport of SREBPs from the endoplasmic reticulum (ER) to the Golgi for proteolytic activation. The SCAP antibody provides an essential tool for studying the molecular regulation of lipid metabolism and cholesterol signaling.\u003cbr\u003e\u003cbr\u003eSREBP cleavage-activating protein contains multiple transmembrane domains and a sterol-sensing domain that binds cholesterol. Under high-cholesterol conditions, SCAP associates with insulin-induced gene (INSIG) proteins in the ER, retaining SREBPs in an inactive state. When sterol levels fall, this interaction is disrupted, allowing SCAP–SREBP complexes to move to the Golgi. There, site-1 and site-2 proteases release the active SREBP transcription factor, which then migrates to the nucleus to induce lipid metabolism genes. The SCAP antibody enables researchers to track this dynamic process, quantifying SCAP expression and subcellular localization under various lipid conditions.\u003cbr\u003e\u003cbr\u003eAs a central regulator of lipid homeostasis, SREBP cleavage-activating protein plays critical roles in energy balance, membrane synthesis, and metabolic adaptation. Dysregulation of SCAP contributes to disorders such as hyperlipidemia, fatty liver disease, obesity, and atherosclerosis. The SCAP antibody supports investigation of these metabolic conditions by detecting protein expression in liver, adipose tissue, and other lipid-synthesizing organs. In cellular studies, it aids analysis of sterol-dependent trafficking and SREBP activation using immunoblotting and immunofluorescence techniques.\u003cbr\u003e\u003cbr\u003eIn addition to cholesterol regulation, SCAP participates in broader metabolic pathways influenced by insulin signaling and nutrient status. Altered SCAP activity has been linked to metabolic reprogramming in cancer cells, where enhanced lipid biosynthesis supports rapid proliferation. The SCAP antibody can be used to study these mechanisms and assess the impact of pharmacologic SCAP inhibition on lipid synthesis and growth control. Western blotting and immunohistochemistry using this antibody reveal SCAP's distribution across the ER and Golgi, providing a spatial understanding of its sterol-sensing function.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SCAP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SCAP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SCAP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SCAP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SCAP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288201581,"sku":"FY12361","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2bc55eab-dfd7-415c-a0fc-fcf66179b18f.jpg?v=1782237002","url":"https:\/\/www.ebiohippo.com\/products\/scap-antibody-srebp-cleavage-activating-protein-bha17135264","provider":"BioHippo","version":"1.0","type":"link"}