{"product_id":"scd1-antibody-stearoyl-coa-desaturase-bha17135424","title":"SCD1 Antibody \/ Stearoyl-CoA desaturase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSCD1 Antibody \/ Stearoyl-CoA desaturase is a anti-SCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: ER.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SCD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSCD1 antibody detects Stearoyl-CoA desaturase, an endoplasmic reticulum enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, primarily oleate and palmitoleate. This reaction is essential for maintaining membrane fluidity, lipid signaling, and triglyceride synthesis. The SCD1 antibody is widely used in metabolism and lipid biology research to explore fatty acid desaturation and metabolic regulation.\u003cbr\u003e\u003cbr\u003eSCD1 is encoded by the SCD gene located on human chromosome 10q24.31. The enzyme is approximately 41 kilodaltons and contains four transmembrane domains that anchor it to the ER membrane, where it introduces a cis-double bond between the ninth and tenth carbon atoms of saturated fatty acyl-CoAs. The desaturation reaction requires oxygen, NADH, and cytochrome b5, forming the core step in the synthesis of monounsaturated fatty acids.\u003cbr\u003e\u003cbr\u003eThe SCD1 antibody typically detects a 36-42 kilodalton band by western blot and reveals reticular ER staining in immunofluorescence microscopy. SCD1 plays a vital role in lipid homeostasis by controlling the ratio of saturated to unsaturated fatty acids, influencing membrane composition, lipoprotein secretion, and energy storage.\u003cbr\u003e\u003cbr\u003eUpregulation of SCD1 promotes lipogenesis and is associated with obesity, insulin resistance, and hepatic steatosis. Conversely, inhibition or genetic deletion of SCD1 enhances fatty acid oxidation and protects against metabolic disease. SCD1 expression is regulated by sterol regulatory element-binding protein 1c (SREBP1c) and peroxisome proliferator-activated receptors (PPARs), linking it to dietary and hormonal control of lipid metabolism.\u003cbr\u003e\u003cbr\u003eBeyond metabolism, SCD1 influences inflammation, cancer cell proliferation, and stem cell maintenance. High SCD1 expression correlates with poor prognosis in several cancers due to its role in sustaining membrane synthesis and redox balance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SCD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SCD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SCD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SCD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SCD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291511149,"sku":"FY12521","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6b12780e-7a61-4ead-9c0b-865029ef0e81.jpg?v=1782237018","url":"https:\/\/www.ebiohippo.com\/products\/scd1-antibody-stearoyl-coa-desaturase-bha17135424","provider":"BioHippo","version":"1.0","type":"link"}