{"product_id":"sclt1-antibody-sodium-channel-and-clathrin-linker-1-bha17135393","title":"SCLT1 Antibody \/ Sodium channel and clathrin linker 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSCLT1 Antibody \/ Sodium channel and clathrin linker 1 is a anti-SCLT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SCLT1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSCLT1 antibody detects Sodium channel and clathrin linker 1, a centrosomal and ciliary base protein essential for ciliogenesis and centrosome organization. SCLT1 participates in the distal appendage complex of centrioles, which anchors the mother centriole to the plasma membrane during primary cilia formation. The SCLT1 antibody is widely used in cell biology and developmental studies to investigate ciliary assembly, centriole architecture, and cilia-dependent signaling pathways.\u003cbr\u003e\u003cbr\u003eSCLT1 is encoded by the SCLT1 gene located on human chromosome 4q28.1. The protein is approximately 68 kilodaltons and is conserved among vertebrates. It contains coiled-coil regions that mediate binding to other distal appendage proteins, including CEP164, FBF1, and CEP83. SCLT1 provides structural linkage between microtubule-based centrioles and the membrane docking machinery required for ciliogenesis initiation. Knockout or mutation of SCLT1 disrupts distal appendage integrity and prevents cilia formation.\u003cbr\u003e\u003cbr\u003eThe SCLT1 antibody detects the protein by western blot as a 68 kilodalton band and reveals punctate centrosomal staining under immunofluorescence microscopy. Localization studies show that SCLT1 is present at the distal end of the mother centriole and co-localizes with ciliary initiation markers. Functional analyses demonstrate that SCLT1 depletion inhibits ciliary vesicle docking, leading to defective Hedgehog signaling and altered tissue morphogenesis. Mutations in SCLT1 have been linked to ciliopathies such as Senior-Loken syndrome and orofaciodigital spectrum disorders, highlighting its importance in organ development.\u003cbr\u003e\u003cbr\u003eBeyond its role in ciliogenesis, SCLT1 may interact with ion channels and clathrin adaptor proteins, potentially linking ciliary membrane organization with endocytic trafficking. It has also been implicated in cell cycle control through centrosome duplication regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SCLT1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SCLT1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SCLT1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SCLT1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SCLT1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290265965,"sku":"FY12490","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fe7af8d6-ec5d-43b4-90d1-d09ac8d9d89d.jpg?v=1782237015","url":"https:\/\/www.ebiohippo.com\/products\/sclt1-antibody-sodium-channel-and-clathrin-linker-1-bha17135393","provider":"BioHippo","version":"1.0","type":"link"}