{"product_id":"scml2-antibody-sex-comb-on-midleg-like-protein-2-bha17136024","title":"SCML2 Antibody \/ Sex comb on midleg-like protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSCML2 Antibody \/ Sex comb on midleg-like protein 2 is a anti-SCML2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SCML2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSCML2 antibody detects Sex comb on midleg-like protein 2, a Polycomb group protein involved in chromatin silencing and transcriptional repression. The UniProt recommended name is Sex comb on midleg-like protein 2 (SCML2). This epigenetic regulator participates in the formation of Polycomb repressive complexes that maintain gene silencing during development and differentiation.\u003cbr\u003e\u003cbr\u003eFunctionally, SCML2 antibody identifies a 715-amino-acid nuclear protein containing SAM and MBT domains that mediate protein-protein and chromatin interactions. SCML2 binds histone tails modified by methylation, such as H3K9me and H4K20me, to recruit other Polycomb factors and repress transcription. It is particularly enriched in testis, suggesting specialized functions in germ cell chromatin organization.\u003cbr\u003e\u003cbr\u003eThe SCML2 gene is located on the X chromosome (Xp22.12) and is expressed in germ cells, embryonic tissues, and certain somatic cells. SCML2 coordinates chromatin compaction and transcriptional silencing by recognizing repressive histone marks and interacting with PRC1 components. It contributes to maintaining stem cell identity and regulating spermatogenesis.\u003cbr\u003e\u003cbr\u003ePathologically, altered SCML2 expression has been linked to developmental defects, infertility, and cancer. Dysregulation may disrupt Polycomb-mediated silencing, leading to aberrant gene expression and loss of epigenetic stability. Research using SCML2 antibody aids in studying chromatin biology, gene silencing, and germline development.\u003cbr\u003e\u003cbr\u003eSCML2 antibody is validated for western blotting, chromatin immunoprecipitation, and immunofluorescence to detect Polycomb group proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Sex comb on midleg-like protein 2 contains a sterile alpha motif (SAM) domain for protein dimerization and two MBT repeats that bind methylated histones. This antibody enables research into SCML2's role in epigenetic repression and chromatin organization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SCML2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SCML2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SCML2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SCML2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SCML2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312613741,"sku":"FY13122","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bac4ee5a-afbc-4835-bb1e-599d9677852f.jpg?v=1782237093","url":"https:\/\/www.ebiohippo.com\/products\/scml2-antibody-sex-comb-on-midleg-like-protein-2-bha17136024","provider":"BioHippo","version":"1.0","type":"link"}