{"product_id":"scrambled-shrna-control-lentivirus-bhv19400139","title":"Scrambled shRNA Control Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eScrambled shRNA control lentiviruses are essential negative controls for RNA interference experiments. A scrambled shRNA carries a sequence that does not match any known mammalian transcript, so it engages the cellular RNAi machinery without silencing a specific gene. Including a scrambled control allows researchers to distinguish true gene-specific phenotypes from non-specific effects of lentiviral transduction, shRNA processing, or selection. Using a control matched to the experimental shRNA system is standard practice and is required for rigorous interpretation of knockdown studies in cancer, cell biology, and functional genomics research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Scrambled shRNA Control Lentivirus is generated from a mixture of two independent scrambled shRNA constructs that do not target any known gene. Like the gene-specific shRNA products, the shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt is recommended as a matched negative control for any shRNA or miRNA lentiviral knockdown experiment, supporting accurate interpretation of gene-specific phenotypes.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting Scrambled (Mix) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251623944557,"sku":"LSV-0024-2L","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311257739629,"sku":"LSV-0024-1L","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 2x10^6","offer_id":53311257772397,"sku":"LSV-0024-1S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 2x10^6","offer_id":53311257805165,"sku":"LSV-0024-2S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311257837933,"sku":"LSV-0024-3S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311257870701,"sku":"LSV-0024-4S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 2x10^6","offer_id":53311257903469,"sku":"LSV-0024-5S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 2x10^6","offer_id":53311257936237,"sku":"LSV-0024-6S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2.5x10^6","offer_id":53311257969005,"sku":"LSV-0024-7S","price":395.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 2.5x10^6","offer_id":53311258001773,"sku":"LSV-0024-8S","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0024-Scrambled-sh-stable-cell-line.jpg?v=1776934219","url":"https:\/\/www.ebiohippo.com\/products\/scrambled-shrna-control-lentivirus-bhv19400139","provider":"BioHippo","version":"1.0","type":"link"}