{"product_id":"sec61b-antibody-sec61-subunit-beta-bha17135263","title":"SEC61B Antibody \/ Sec61 subunit beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSEC61B Antibody \/ Sec61 subunit beta is a anti-SEC61B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SEC61B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SEC61B antibody targets Protein transport protein Sec61 subunit beta, a component of the Sec61 translocon complex encoded by the SEC61B gene. The Sec61 complex mediates the translocation of nascent polypeptides across and into the endoplasmic reticulum (ER) membrane, an essential step in the biosynthesis of secretory and membrane proteins. The SEC61B antibody enables precise detection of this translocon subunit and supports studies of protein targeting, membrane insertion, and ER homeostasis.\u003cbr\u003e\u003cbr\u003eProtein transport protein Sec61 subunit beta forms part of the heterotrimeric Sec61 channel, together with the alpha (SEC61A) and gamma (SEC61G) subunits. While the alpha subunit forms the central pore that guides polypeptides through the membrane, the beta subunit participates in channel assembly, stability, and interaction with ribosomes and chaperones. The SEC61B antibody provides a reliable means of detecting this small yet essential component of the translocon, helping researchers understand how it contributes to co-translational protein translocation and membrane integration.\u003cbr\u003e\u003cbr\u003eIn addition to its structural role, Protein transport protein Sec61 subunit beta is involved in post-translational translocation of proteins and quality control mechanisms that prevent accumulation of misfolded peptides. The SEC61B antibody can be used to monitor ER function under stress conditions, including the unfolded protein response and ER-associated degradation (ERAD). By visualizing SEC61B distribution and expression levels, investigators gain insights into how protein import efficiency correlates with ER capacity and cell health.\u003cbr\u003e\u003cbr\u003eThe Sec61 complex also functions as a retrotranslocation channel, allowing misfolded proteins to exit the ER for cytosolic degradation by the proteasome. This dual functionality links SEC61B to cellular proteostasis and stress adaptation. The SEC61B antibody is instrumental in exploring these mechanisms and clarifying how perturbations in Sec61 function contribute to diseases such as cystic fibrosis, diabetes, and neurodegeneration. Its detection of Protein transport protein Sec61 subunit beta expression enables both basic and translational research on ER transport regulation.\u003cbr\u003e\u003cbr\u003eThe SEC61B antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry. It produces distinct ER localization patterns consistent with translocon distribution.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SEC61B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SEC61B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SEC61B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SEC61B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SEC61B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289053549,"sku":"FY12360","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c71f0eb3-6524-4e78-ae2a-ec0b01511031.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/sec61b-antibody-sec61-subunit-beta-bha17135263","provider":"BioHippo","version":"1.0","type":"link"}