{"product_id":"secisbp2-antibody-selenocysteine-insertion-sequence-binding-protein-2-bha17136265","title":"SECISBP2 Antibody \/ Selenocysteine insertion sequence-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSECISBP2 Antibody \/ Selenocysteine insertion sequence-binding protein 2 is a anti-SECISBP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nucleus, Miochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SECISBP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSECISBP2 antibody detects Selenocysteine insertion sequence-binding protein 2, a cytoplasmic RNA-binding protein encoded by the SECISBP2 gene located on chromosome 9q22.2. SECISBP2 is essential for the synthesis of selenoproteins, a unique class of proteins that incorporate the amino acid selenocysteine at UGA codons during translation. The protein binds specifically to selenocysteine insertion sequence (SECIS) elements in the 3' untranslated regions (3' UTRs) of selenoprotein mRNAs, promoting selenocysteine incorporation by interacting with specialized translation factors. SECISBP2 is widely expressed, with high levels in liver, thyroid, and kidney where selenoprotein synthesis is most active.\u003cbr\u003e\u003cbr\u003eStructurally, SECISBP2 contains RNA recognition motifs and a lysine-rich domain essential for binding SECIS elements. It forms part of a ribonucleoprotein complex with eukaryotic elongation factor EEFSEC and selenocysteine-specific tRNA (tRNA-Sec), ensuring accurate decoding of UGA as selenocysteine instead of a stop signal. SECISBP2 belongs to the SECIS-binding protein family, which includes SECISBP2L, a paralog with partially overlapping function. Co-localization studies show SECISBP2 distributed in cytoplasmic foci associated with ribosomes and endoplasmic reticulum-bound polysomes, indicating its role in co-translational selenoprotein synthesis.\u003cbr\u003e\u003cbr\u003eFunctionally, SECISBP2 regulates the expression of numerous selenoproteins involved in antioxidant defense, redox regulation, and thyroid hormone metabolism. These include glutathione peroxidases, thioredoxin reductases, and deiodinases. By controlling selenocysteine incorporation efficiency, SECISBP2 influences cellular responses to oxidative stress and selenium availability. During development, SECISBP2 expression supports organogenesis and metabolic maturation by maintaining proper selenoprotein production.\u003cbr\u003e\u003cbr\u003eMutations in SECISBP2 cause a multisystem disorder known as selenoprotein deficiency syndrome, characterized by impaired thyroid hormone activation, low plasma selenium levels, and muscle weakness. Reduced SECISBP2 function decreases synthesis of key antioxidant enzymes, leading to increased oxidative damage. Conversely, overexpression can enhance cellular resistance to oxidative stress by upregulating selenoprotein synthesis. Pathway associations include selenoamino acid metabolism, translational control, and oxidative stress response. Known binding partners include EEFSEC, SEPSECS, and tRNA-SEC, forming the machinery required for selenocysteine translation.\u003cbr\u003e\u003cbr\u003eThe SECISBP2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SECISBP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SECISBP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SECISBP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SECISBP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SECISBP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323820397,"sku":"FY13363","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_83aec02d-4107-42fc-a804-67a42b95140a.jpg?v=1782237114","url":"https:\/\/www.ebiohippo.com\/products\/secisbp2-antibody-selenocysteine-insertion-sequence-binding-protein-2-bha17136265","provider":"BioHippo","version":"1.0","type":"link"}